Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Combined Automated Annotation using Multiple IEA Methods
Characterization of native and recombinant bone sialoprotein: delineation of the mineral-binding and cell adhesion domains and structural analysis of the RGD domain.
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Native bone sialoprotein binds hydroxyapatite with nanomolar affinity.
"hydroxyapatite Kd approximately 2.6 x 10(-9) M"
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Native BSP and an internal fragment inhibit seeded hydroxyapatite crystal growth.
"Both native BSP and a 47 kD fragment of UMR-BSP"
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BSP supports adhesion through RGD and non-RGD regions.
"BSP facilitates the adhesion of several cell types through its integrin binding (RGD) tripeptide sequence."
Factor H binding to bone sialoprotein and osteopontin enables tumor cell evasion of complement-mediated attack.
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BSP forms rapid, tight complexes with complement factor H.
"BSP and OPN form rapid and tight complexes with complement Factor H."
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Recombinant BSP protects cells from complement-mediated attack.
"Recombinant OPN and BSP can protect murine erythroleukemia cells from attack by human complement"
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BSP first engages alpha-V/beta-3 and then recruits factor H to the cell surface.
"initial binding to alpha(V)beta(3) integrin"
Activation of integrin alpha(V)beta(3) regulates cell adhesion and migration to bone sialoprotein.
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Activated alpha-V/beta-3 mediates adhesion of multiple human cell types to BSP.
"markedly enhanced alpha(V)beta(3)-dependent adhesion to BSP."
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BSP supports alpha-V/beta-3-mediated cell migration.
"alpha(V)beta(3)-mediated migration of HUVEC or osteoblastic cells to BSP was substantially enhanced"
Structural characterization of human recombinant and bone-derived bone sialoprotein. Functional implications for cell attachment and hydroxyapatite binding.
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Full-length recombinant human and native bone-derived BSP were purified and characterized.
"produced full-length recombinant BSP in a human cell line and purified the protein from human bone"
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Native human bone BSP has higher hydroxyapatite affinity than recombinant BSP.
"The affinity for hydroxyapatite was higher for bone-derived BSP than for recombinant BSP."
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Human BSP supports cell adhesion in a conformation-dependent manner.
"Cell adhesion assays showed that the binding of BSP to cells can be reversibly diminished by denaturation."
Differential expression profiling of membrane proteins by quantitative proteomics in a human mesenchymal stem cell line undergoing osteoblast differentiation.
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The study measured membrane-associated proteomes during short-term osteoblast differentiation.
"used MS to characterize changes in expression of membrane protein markers before and after short-term induction of osteoblast"
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The abstract does not expose the IBSP-specific measurement.
"We identified 463 unique proteins with extremely high confidence"
Expression of bone sialoprotein (BSP) in developing human tissues.
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BSP protein and transcript occur in mature bone-forming cells but not immature precursors.
"Both protein and mRNA were found in mature, bone-forming cells but not in their immature precursors."
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BSP is an osteoblast-derived component of human bone matrix.
"BSP is primarily an osteoblast-derived component of the bone matrix expressed at late stages of differentiation."
Matrix vesicles are carriers of bone morphogenetic proteins (BMPs), vascular endothelial growth factor (VEGF), and noncollagenous matrix proteins.
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Rat growth-plate matrix vesicles contain BSP.
"BSP, ON, OPN, and OC were all detected by Western blot analyses."
Structure-activity relationship of human bone sialoprotein peptides.
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An RGD-containing human BSP peptide supports osteoblast-like cell attachment.
"attachment activity toward osteoblast-like (MC3T3) cells"
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Replacing RGD with KAE dramatically reduces cell attachment.
"replacement of the RGD (arg-gly-asp) peptide moiety with peptide KAE (lys-ala-glu) resulted in a dramatic loss of cell-attachment activity"
Skeletal development of mice lacking bone sialoprotein (BSP)--impairment of long bone growth and progressive establishment of high trabecular bone mass.
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Ibsp-null newborn mice have delayed primary ossification and reduced cortical mineral density.
"In BSP-/- newborns, µCT analysis revealed a delay in membranous primary ossification"
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Loss of BSP alters primary bone formation and mineralization.
"lack of BSP alters long bone growth and membranous/cortical primary bone formation and mineralization."
A reference map of the human binary protein interactome.
A molecular interactome of the glioblastoma perivascular niche reveals integrin binding sialoprotein as a mediator of tumor cell migration.
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Functional assays and mouse models show that IBSP promotes glioblastoma tumor-cell growth and vascular migration.
"IBSP, enhances tumor growth and promotes the migration of GTCs along the vasculature."
MatrisomeDB 2.0: 2023 updates to the ECM-protein knowledge database.
Interaction of integrin alphaVbeta3 with IPSP (Bone sialoprotein 2)
UniProtKB record for human IBSP (P21815)
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IBSP is a reviewed 317-amino-acid secreted precursor.
"Reviewed; 317 AA."
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UniProt records an N-terminal signal peptide.
"FT SIGNAL 1..16"
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UniProt records the RGD integrin-binding motif.
"FT MOTIF 286..288"
Manual literature and annotation review notes for human IBSP
Manual literature synthesis for human IBSP (P21815)
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IBSP couples hydroxyapatite binding with RGD-dependent integrin binding in mineralized matrix.
"IBSP is best understood as a mineralized-matrix ligand with two coupled molecular activities: hydroxyapatite binding and RGD-dependent integrin binding."