Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
The ArfGEF GBF-1 Is Required for ER Structure, Secretion and Endocytic Transport in C. elegans.
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GBF-1 localizes to the cis face of the Golgi at transitional ER-Golgi elements in oocytes.
"GBF-1 is present on the cis-face of the Golgi at so-called transitional ER-Golgi elements"
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gbf-1 RNAi causes vesicles to accumulate at the cis-Golgi and the Golgi mini-stacks to aggregate.
"upon gbf-1(RNAi) numerous vesicles accumulated at the cis-Golgi, representing most likely ER-derived COPII vesicles"
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gbf-1 RNAi disrupts ER structure in oocytes. The authors consider this indirect.
"In gbf-1(RNAi) oocytes we observed a severely disturbed ER organization"
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The secretion defects are thought to be secondary to impaired retrograde transport.
"However, this role is likely indirect through GBF-1's involvement in retrograde transport to the ER."
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The null mutant is lethal.
"Since the null mutant in gbf-1 is lethal"
The small GTPase Arf1 modulates mitochondrial morphology and function.
Serum- and Glucocorticoid-Inducible Kinase-1 (SGK-1) Plays a Role in Membrane Trafficking in Caenorhabditis elegans.
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GBF-1 co-immunoprecipitates with SGK-1, and gbf-1 RNAi disrupts basolateral SGK-1::GFP in the intestine.
"Considering that GBF-1 co-immunoprecipitated with SGK-1 (Table 1), this result suggests that GBF-1 may be required for SGK-1 function near the basolateral membrane"
UniProt entry G5EGS5 (GBF1_CAEEL)
gbf-1 manual literature review