Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Combined Automated Annotation using Multiple IEA Methods
Identification of the mstE gene encoding a glucose-inducible, low affinity glucose transporter in Aspergillus nidulans.
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MstE is the low-affinity glucose transporter active in conidia germinating on glucose.
"The mstE gene encoding a low affinity glucose transporter active during the germination of Aspergillus nidulans conidia on glucose medium has been identified"
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mstE expression in hyphae is induced by repressing carbon sources and requires CreA.
"mstE expression also occurs in hyphae, is induced in the presence of other repressing carbon sources besides glucose, and is dependent on the function of the transcriptional repressor CreA"
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MstE-sGFP localises to the cell periphery, consistent with the plasma membrane.
"fluorescence at the periphery of conidia and hyphae is consistent with MstE location in the plasma membrane"
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Deleting mstE abolishes low-affinity glucose uptake without a morphological phenotype.
"Deletion of mstE has no morphological phenotype but results in the absence of low affinity glucose uptake kinetics, the latter being substituted by a high affinity system"
High-affinity glucose transport in Aspergillus nidulans is mediated by the products of two related but differentially expressed genes.
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MstE is the established low-affinity glucose uptake system of A. nidulans; MstA and MstC are high-affinity paralogs.
"Until very recently, the only A. nidulans monosaccharide uptake system to have been characterised biochemically, physiologically and genetically was that of the low-affinity glucose transporter encoded by mstE - genomic locus AN5860"
Identification of glucose transporters in Aspergillus nidulans.
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The A. nidulans paralogs HxtB, HxtC and HxtE showed CCCP-sensitive glucose uptake in yeast, suggesting proton symport; MstE was not tested.
"Taken together, these data suggested that HxtB, HxtC, and HxtE mediated glucose transport via active proton symport."
The low affinity glucose transporter HxtB is also involved in glucose signalling and metabolism in Aspergillus nidulans.
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mstE is among the most strongly down-regulated transporter genes in low glucose.
"Genes encoding the two transporters AtrA and MstE, presented the highest down-regulation"
Identification and characterization of the glucose dual-affinity transport system in Neurospora crassa: pleiotropic roles in nutrient transport, signaling, and carbon catabolite repression.
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The SF92 ortholog GLT-1 of N. crassa is likewise a low-affinity glucose transporter.
"we assigned GLT-1 (Km = 18.42 ± 3.38 mM) and HGT-1/-2 (Km = 16.13 ± 0.95 and 98.97 ± 22.02 µM) to the low- and high-affinity glucose transport systems, respectively"
RCO-3 and COL-26 form an external-to-internal module that regulates the dual-affinity glucose transport system in Neurospora crassa.
MstE PANTHER/PAINT placement and TM1 protonation-site analysis
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MstE and N. crassa GLT-1 are orthologs that meet at the Pezizomycotina speciation node at the root of PTHR48022:SF92.
"orthologs within SF92, separated by no duplication."
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MstE is not a seed of the family-root carbohydrate:proton symporter IBD.
"and GO:0005351 carbohydrate:proton symporter activity (MstE is NOT a seed; seeds are"