Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs using Ensembl Compara
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Gene Ontology annotation based on curation of immunofluorescence data
Automatic assignment of GO terms using logical inference, based on inter-ontology links
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Identification of a novel isopeptidase with dual specificity for ubiquitin- and NEDD8-conjugated proteins.
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USP21 is a cysteine-protease isopeptidase that removes ubiquitin from ubiquitinated proteins and removes NEDD8 from NEDD8 conjugates, but has no effect on Sentrin-1/SUMO conjugates (dual ubiquitin/NEDD8 specificity).
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Catalytic Cys-221 is required; its mutation abolishes ubiquitin thioesterase activity.
Defining the human deubiquitinating enzyme interaction landscape.
A global survey of CRM1-dependent nuclear export sequences in the human deubiquitinase family.
A proteome-scale map of the human interactome network.
BEND3 represses rDNA transcription by stabilizing a NoRC component via USP21 deubiquitinase.
Distinct regulatory ribosomal ubiquitylation events are reversible and hierarchically organized.
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USP21 (with OTUD3) is a deubiquitylating enzyme that antagonizes ZNF598-mediated 40S ribosomal ubiquitylation (on eS10/RPS10 and uS10/RPS20) and limits ribosome-associated quality control (RQC) activation.
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Cells lacking USP21 have altered RQC activity and delayed eS10 deubiquitylation, establishing a functional role for USP21 within the RQC pathway.
A reference map of the human binary protein interactome.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
Ub-specific processing proteases (protein deubiquitination)
Ub-specific processing proteases (USP21 deubiquitinase activity)
Ub-specific processing proteases (USP21 deubiquitinase activity)
Ub-specific processing proteases (USP21 deubiquitinase activity)
Falcon deep research report for USP21
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USP21 is a broad-specificity DUB that processes multiple polyubiquitin chain types (K6, K11, K29, K48, K63, and linear) and cross-reacts with ISG15, with substrate-stabilizing deubiquitination reported for RIG-I (K63), MEK2 (K48), Nanog (K48), histone H2A (H2AK119ub), MARK3, TCF7, GATA3 and RIPK1.