Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Role of flagella in pathogenesis of Pseudomonas aeruginosa pulmonary infection.
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fliC disruption mutants of strain PAK were constructed by gene replacement and identified as nonmotile.
"Nonmotile mutants of PAK were analyzed to confirm that the expected gene replacement events, in which the interrupted copy of fliC , the gene encoding flagellin protein, introduced into PAK and PAK/NP had replaced the wild-type coding sequence, had occurred."
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The fliC mutants were made in strain PAK, not PAO1.
"Fla − mutants of PAK and PAK/NP were isolated by gene replacement."