Use of the ND evidence code for Gene Ontology (GO) terms
Annotation inferences using phylogenetic trees
Role of a class DHC1b dynein in retrograde transport of IFT motors and IFT raft particles along cilia, but not dendrites, in chemosensory neurons of living Caenorhabditis elegans.
-
OSM-6 is a homologue of a Chlamydomonas 16S IFT raft (IFT particle) polypeptide and, together with OSM-1 and kinesin-II, undergoes retrograde IFT along cilia at ~1.1 um/s; retrograde ciliary transport requires the CHE-3 (DHC1b/dynein-2) motor.
"OSM-1 and OSM-6, all move at approximately 1.1 microm/s in the retrograde direction along cilia and dendrites"
Genetic analysis of chemosensory control of dauer formation in Caenorhabditis elegans.
-
osm-6 is one of nine cilium-structure genes whose mutation causes structurally defective chemosensory cilia and blocks chemosensation, placing it at a single step in the dauer-formation epistasis pathway.
"Dauer-defective mutations in nine genes cause structurally defective chemosensory cilia, thereby blocking chemosensation."
Endocytosis genes facilitate protein and membrane transport in C. elegans sensory cilia.
-
OSM-6 is used as an IFT/ciliary marker in C. elegans sensory cilia; the study examines transport of ciliary and periciliary membrane and IFT proteins.
"These pathways include cilium-based intraflagellar transport (IFT) and poorly understood membrane trafficking events."
The BBSome controls IFT assembly and turnaround in cilia.
-
GFP-tagged OSM-6 is the canonical IFT-B reporter (explicitly the ortholog of human IFT52) used to screen for IFT assembly/turnaround mutants; OSM-6 marks IFT particles at the ciliary base and along cilia.
"the GFP-tagged IFT-B component OSM-6 (the ortholog of human IFT52)"
Neuropeptide signaling remodels chemosensory circuit composition in Caenorhabditis elegans.
-
Hypomorphic osm-6 mutants lack functional sensory cilia and consequently show greatly reduced salt (ASEL) and salt/odor (AWC) sensory responses; ciliary function is restored cell-autonomously by osm-6 rescue.
"We analyzed hypomorphic osm-6 mutants that lack functional sensory cilia (dendritic endings) and found them to have greatly reduced ASEL salt responses and AWCON salt and odor responses"
Mutant sensory cilia in the nematode Caenorhabditis elegans.
-
osm-6(p811) mutants have normal ciliary transition zones but severely shortened axonemes with ectopic doublet microtubules, establishing OSM-6 as required for axoneme (cilium) assembly rather than transition-zone formation.
"The cilia in che-13 (e1805), osm-1 (p808), osm-5 (p813), and osm-6 (p811) mutants have normal transition zones and severely shortened axonemes."
Whole-Organism Developmental Expression Profiling Identifies RAB-28 as a Novel Ciliary GTPase Associated with the BBSome and Intraflagellar Transport.
-
OSM-6::GFP (explicitly the IFT52 orthologue) localizes throughout normal-length sensory cilia and is used as an established IFT/ciliary marker.
"Localisation of an IFT protein, OSM-6 (IFT52 orthologue), throughout normal-length cilia confirms this finding"
Dynein-Driven Retrograde Intraflagellar Transport Is Triphasic in C. elegans Sensory Cilia.
-
Ciliary entry of dynein-2 requires an intact IFT-B complex (of which OSM-6/IFT52 is a component); this study is the ComplexPortal source (CPX-1290) for OSM-6 IFT-B/IFT particle B membership and ciliary localization.
"Disruption of the dynein-2 tail domain, light intermediate chain, or intraflagellar transport (IFT)-B complex abolishes dynein-2's ciliary localization"
Osmotic avoidance defective mutants of the nematode Caenorhabditis elegans.
-
Founding screen that isolated osmotic-avoidance-defective (Osm) mutants of C. elegans, the phenotypic class for which osm-6 is named; such mutants fail to avoid high concentrations of salts/sugars.
"mutants were selected for their inability to avoid high concentrations of fructose or NaCl"
A dual mechanosensory and chemosensory neuron in Caenorhabditis elegans.
-
Animals with defective ciliated sensory endings fail to respond to nose touch, linking intact sensory cilia (which require osm-6) to mechanosensation.
"Mutant animals that have defective ciliated sensory endings as well as laser-operated animals that lack ASH, FLP, and OLQ fail to respond to touch to the nose."
Analysis of osm-6, a gene that affects sensory cilium structure and sensory neuron function in Caenorhabditis elegans.
-
osm-6 was cloned and shown to encode a protein of previously unknown function expressed exclusively in ciliated sensory neurons; OSM-6::GFP rescues the mutant and localizes to cytoplasm/processes/dendritic endings, and osm-6 acts cell-autonomously in cilium structure. DAF-19 is required for OSM-6 accumulation.
"The OSM-6::GFP protein was localized to cytoplasm, including processes and dendritic endings where sensory cilia are situated."
-
Genetic mosaic analysis shows osm-6 acts cell-autonomously in the ciliated neuron.
"we conclude from an analysis of genetic mosaics that osm-6 acts cell autonomously in affecting cilium structure"