Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Two genetically distinct methyl-coenzyme M reductases in Methanobacterium thermoautotrophicum strain Marburg and delta H.
-
Two genetically distinct MCR isoenzymes (MCR I and MCR II), each composed of alpha, beta and gamma subunits, were purified from strains Marburg and Delta H; MCR I predominates under gas-limited growth.
"When growth was limited by the gas supply, MCR I predominated."
Evidence that the heterodisulfide of coenzyme M and 7-mercaptoheptanoylthreonine phosphate is a product of the methylreductase reaction in Methanobacterium.
-
In Delta H cell extracts, coenzyme B reduces methyl-coenzyme M to methane with stoichiometric formation of CoM-S-S-CoB heterodisulfide.
"CH4 and CoM-S-S-HTP were produced stoichiometrically in a ratio of 1:1."
The final step in methane formation. Investigations with highly purified methyl-CoM reductase (component C) from Methanobacterium thermoautotrophicum (strain Marburg).
Crystal structure of methyl-coenzyme M reductase: the key enzyme of biological methane formation.
The radical mechanism of biological methane synthesis by methyl-coenzyme M reductase.
Methyl (Alkyl)-Coenzyme M Reductases: Nickel F-430-Containing Enzymes Involved in Anaerobic Methane Formation and in Anaerobic Oxidation of Methane or of Short Chain Alkanes.
Growth phase-dependent transcription of the genes that encode the two methyl coenzyme M reductase isoenzymes and N5-methyltetrahydromethanopterin:coenzyme M methyltransferase in Methanobacterium thermoautotrophicum delta H.
-
mcrA is in the mcrBDCGA operon upstream of mtr; mcr transcription replaces mrt (MCR II) transcription later in batch growth.
"Transcription of the mrt operon was found to occur only at early times in batch cultures and was then replaced by transcription of the mcr operon."
Post-translational modifications in the active site region of methyl-coenzyme M reductase from methanogenic and methanotrophic archaea.
Phylogenetic and Structural Comparisons of the Three Types of Methyl Coenzyme M Reductase from Methanococcales and Methanobacteriales.
UniProt entry O27232 (MCRA_METTH)