Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
Pathway for lipid A biosynthesis in Arabidopsis thaliana resembling that of Escherichia coli.
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atlpxb-1 and atlpxb-2 null mutants accumulate the LpxB substrates lipid X (42-fold) and UDP-2,3-diacyl-GlcN, but have no visible phenotype.
"Lipid X accumulates 42-fold in the atlpxb-1 mutant compared to the Col-0 wild type by this criterion"
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A 5'-terminal AtLpxB-GFP fusion is targeted to mitochondria; the full-length fusion was too dim to localise.
"Full-length GFP fusions of AtLpxD1, AtLpxD2, AtLpxB, and AtLpxK did not emit sufficient GFP fluorescence to permit localization."
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The lipid X pool of lpxb-1 plants is enriched in mitochondria.
"Lipid X levels in mitochondria were 3- and 48-fold higher than in chloroplasts or whole cell homogenates, respectively."
Detecting Gene Ontology misannotations using taxon-specific rate ratio comparisons.
UniProt entry F4IF99 (LPXB_ARATH)