cyaB (PA3217, Q9HZ23) review notes

Class IIIb adenylyl cyclase with an N-terminal MASE2 membrane sensor domain (six
predicted TM helices, Phobius) and a C-terminal Guanylate_cyc (CHD) catalytic domain.

Literature (no deep-research provider available; own reading)

Wolfgang et al. 2003 (abstract only; no PMC full text): microarray work on the
TTSS regulon identified a membrane-associated adenylate cyclase signalling
pathway PMID:12586068; mutants unable to make cAMP were profiled PMID:12586068. This is the PseudoCAP source for the IMP MF and
BP rows.
Topal et al. 2012 (full text, PMC3269522): biochemical and structural
characterisation of CyaB. Purified catalytic domain is active and stimulated by
CO2/HCO3- PMID:22226839;
CyaB makes most cellular cAMP PMID:22226839; the MASE2 domain anchors it in the inner
membrane PMID:22226839. This gives
direct evidence for GO:0004016 and a new plasma membrane location row.
GO:0019933 cAMP-mediated signaling is obsolete, so the IEA intracellular signal
transduction row is kept as is (non-core).
PubMed MCP search returned API errors on 2026-11-05.

PseudoCAP IBA-seed assessment

Seed support queried live in QuickGO on 2026-11-05.

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN002454020	GO:0004016 adenylate cyclase activity	12	158 (20 taxa)	GO:0004016, IMP, PMID:12586068 (PseudoCAP)	SOUND	Abstract describes cAMP-null mutants and a membrane adenylate cyclase; the activity is confirmed with purified CyaB in PMID:22226839 (full text). MF by IMP is weaker than IDA but correct.	M. tuberculosis Rv1264, Rv1318c, Rv1319c, Rv1647 (IDA), T. brucei receptor ACs (6, IDA PMID:11166383), Dictyostelium AcrA (IDA)	Yes; eleven IDA seeds
PTN002454020	GO:0006171 cAMP biosynthetic process	4	158 (20 taxa)	GO:0006171, IMP, PMID:12586068 (PseudoCAP)	SOUND	Loss of cAMP synthesis in the mutant; CyaB makes most cellular cAMP (PMID:22226839).	Rv1318c, Rv1319c, Rv1647 (IDA)	Yes; three IDA seeds

No PSEUDOCAP or GO_CENTRAL defect. The node is a class III AC clade; both terms suit it.