Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3'-5')-cyclic-GMP in virulence.
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Extracts of a PA14 strain overexpressing PA3343 contained 22 pmol c-di-GMP per mg wet cell weight (Table 1, read in PMC full text; detection limit 12).
"Analysis of the phenotypes of DGC and PDE mutants and overexpressing clones revealed that certain virulence-associated traits are controlled by multiple DGCs and PDEs through alterations in c-di-GMP levels."
The Diguanylate Cyclase HsbD Intersects with the HptB Regulatory Cascade to Control Pseudomonas aeruginosa Biofilm and Motility.
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HsbD expression causes Congo-red staining in E. coli and a GGAAF active-site mutant does not.
"HsbD causes the red colony staining, phenotype correlated with a global increase in intracellular c-di-GMP levels [30, 31]; while a catalytic inactive GGAAF mutant does not trigger such output."
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hsbD is required for the hyperbiofilm phenotype of an hptB mutant (complemented).
"Finally, complementation of the hsbD deletion restored the hyper-biofilm phenotype of the hptB mutant (S2 Fig)."
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Deleting hsbD in an hptB mutant causes hyper-swarming.
"the resulting strain displays a striking hyper-swarming phenotype (~9 fold increase in swarming area, Fig 4B)."
Phenotypic and integrated analysis of a comprehensive Pseudomonas aeruginosa PAO1 library of mutants lacking cyclic-di-GMP-related genes.
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A PAO1 hsbD deletion slightly increases attachment.
"Three of the mutants in a putative dgc gene resulted in a slight upregulation of attachment (fold change ranging between 1.2 and 1.4): PA2771 (dcsbis; Chen et al., 2016), PA3343 (hsbD; Valentini et al., 2016) and most significantly PA4396."
Gene Ontology annotation based on Enzyme Commission mapping
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt