ELO2 (FEN1/GNS1/VBM2, YCR034W; UniProt P25358) notes

Evidence journal (no paid deep research run; built from UniProt and cached publications).

Function

• ELO-family 3-keto-acyl-CoA synthase (condensing enzyme), first and rate-limiting step of the ER VLCFA elongation cycle; EC 2.3.1.199 [UniProt:P25358].
• Elongase II: PMID:12684876; PMID:12684876.
• PMID:9211877; elo2 elo3 double mutant synthetic lethal PMID:9211877.
• In vitro reconstitution: PMID:17719544; active site faces cytosol, chain-length lysine near luminal end of TM helix PMID:17719544.
• fen1 membranes impaired in elongation beyond C18-CoA PMID:23325927.
• Co-IP with Tsc13 PMID:11113186.

Location

• ER membrane: PMID:9832547; Elo2-GFP ER pattern PMID:11113186.

Indirect phenotypes (curated as over-annotation)

• v-SNARE bypass in secretion (VBM2) PMID:9832547.
• Endosomal trafficking synthetic defects with vps21 PMID:23325927.

Curation decisions

• Cytosol (RCA, PWY-5080-1) removed: integral ER membrane protein.
• IBA PUFA elongation removed (S. cerevisiae has no PUFAs); IBA MUFA elongation marked over-annotated.
• Sphingolipid biosynthesis kept as non-core (precursor supply).