Analysis of Pseudomonas aeruginosa diguanylate cyclases and phosphodiesterases reveals a role for bis-(3'-5')-cyclic-GMP in virulence.
Autolysis and autoaggregation in Pseudomonas aeruginosa colony morphology mutants.
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wspR insertions suppress the wrinkled colony phenotype of a wspF mutant and restore motility; no c-di-GMP or enzyme assay is reported.
"Mutation of wspR completely suppressed colony wrinkledness and restored swarming and swimming motility"
A chemosensory system that regulates biofilm formation through modulation of cyclic diguanylate levels.
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Purified WspR synthesises c-di-GMP in vitro, stimulated by phosphorylation.
"We determined that purified WspR catalyzed the formation of c-diGMP in vitro and phosphorylation stimulated this activity."
Phosphorylation-independent regulation of the diguanylate cyclase WspR.
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WspR activity is modulated by product-driven changes in oligomeric state.
"By switching from an active to a product-inhibited dimer via a tetrameric assembly, WspR utilizes a novel mechanism for modulation of its activity through oligomerization."
Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt