Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
UniProtKB record A0A0H2M784 (A0A0H2M784_VARPD) - Putative aliphatic sulfonates-binding protein from Variovorax paradoxus TBEA6
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VPARA_09270 (ssuA2, A0A0H2M784) is a 319-aa aliphatic sulfonate-binding protein (NCBIfam TIGR01728, InterPro IPR010067, PANTHER PTHR30024:SF42) carrying an N-terminal Tat signal peptide (PROSITE PS51318, residues 1-29).
"FT SIGNAL 1..29
FT /evidence="ECO:0000256|SAM:SignalP""
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VPARA_09270 (ssuA2) corresponds to EMBL CDS accession KLN57916.1 on genomic contig JZWI01000005, where consecutive CDS accessions and locus tags stepping by 10 place ssuA2 in the same organosulfonate gene cluster as ssuB2 (VPARA_09230, KLN57912.1), ssuC5 (VPARA_09240, KLN57913.1), ssuD (VPARA_09250, KLN57914.1), and ssuE (VPARA_09290, KLN57918.1).
"DR EMBL; JZWI01000005; KLN57916.1; -; Genomic_DNA."
Deletion analysis of the Escherichia coli taurine and alkanesulfonate transport systems.
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In-frame deletion and complementation analysis of the E. coli ssuABC and tauABC genes established that SsuABC forms a dedicated ABC importer for aliphatic sulfonates whose subunits are not functionally exchangeable with TauABC.
"Mutants in which only formation of hybrid transporters was possible were unable to grow with sulfonates, indicating that the individual components of the two transport systems were not functionally exchangeable."
The ssu locus plays a key role in organosulfur metabolism in Pseudomonas putida S-313.
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Sequence and complementation analysis of the ssu operon identified ssuA, ssuB, and ssuC as the periplasmic binding, ATPase, and membrane permease genes of an ABC-type sulfonate transport system.
"The first group consists of ssuA , ssuB , and ssuC , which are the genes for a putative ATP-binding cassette (ABC)-type transport system."