Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Functional second genes generated by retrotransposition of the X-linked ribosomal protein genes.
Lys53 of ribosomal protein L36AL and the CCA end of a tRNA at the P/E hybrid site are in close proximity on the human ribosome.
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RPL36AL Lys53 contacts the CCA end of deacylated P/E-state tRNA in human ribosomes.
"the crosslink was formed most efficiently with C74 and C75 of P/E-tRNA, but could also connect the ultimate A of this tRNA with Lys53 of protein RPL36AL"
The CCA-end of P-tRNA Contacts Both the Human RPL36AL and the A-site Bound Translation Termination Factor eRF1 at the Peptidyl Transferase Center of the Human 80S Ribosome.
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Recombinant human RPL36AL binds tRNA with nanomolar affinity and endogenous RPL36AL contacts P-site tRNA in human 80S ribosomes.
"The KD values in the nanomolar range reflect high tRNA-binding affinities with full length and truncated tRNA species."
A proteome-scale map of the human interactome network.
E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
Functional involvement of a conserved motif in the middle region of the human ribosomal protein eL42 in translation.
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Conserved human eL42 residues contribute to proper elongation-cycle progression during deacylated tRNA release from the E site.
"cooperative interactions implicating the eL42 residues Q45, Q51, and K53 play a critical role in the ability of the human ribosome to perform properly elongation cycle at the step of deacylated tRNA dissociation from the E site in the human cell."
UniProtKB record for human RPL36AL (Q969Q0)
Manual deep research synthesis for human RPL36AL
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The direct evidence supports a ubiquitously expressed RPL36AL protein that binds tRNA as a component of the cytosolic 60S subunit without establishing paralog-specific ribosome specialization.
"RPL36AL is a ubiquitously expressed autosomal eL42 paralog whose directly demonstrated molecular activity is tRNA binding within the cytosolic 60S ribosomal subunit."
Manual RPL36AL curation notes
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Records annotation decisions, evidence boundaries, paralog-specific caveats, automated-research failures, and experimental priorities.
"The defensible core is a cytosolic 60S-subunit structural role coupled to direct tRNA binding."