DFR1 (YOR236W, P07807) notes

• DHFR, EC 1.5.1.3 [UniProt:P07807]. "DHFR was overproduced about 40-fold in S. cerevisiae when the cloned gene was present in the vector YEp24." PMID:2838385
• Multicopy DFR1 gives methotrexate resistance PMID:2827121.
• The dfr1 mutant needs dTMP, adenine, histidine and methionine, cannot use folinic acid in YEPD, and is respiratory-deficient PMID:1427091.
• Location: "dihydrofolate reductase (DHFR) ... is present in both the cytoplasm and mitochondria" (citing Zelikson & Luzzati 1977) PMID:15169867. GFP shows cytoplasm and mitochondria PMID:14562095.
• Dfr1p was among the candidate RNA-binding enzymes in a protein-array screen PMID:21124907.

Curation decisions

• The core annotations are GO:0004146 and GO:0046654.
• The mitochondrion IBA and HDA rows are accepted.
• mRNA binding, NADP binding and folic acid metabolic process are kept as non-core.
• The folate cycle row is marked over-annotated.
• The chorismate row is removed.
• The folic acid biosynthetic row is changed to GO:0046654.