Gene Ontology annotation through association of InterPro records with GO terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic transfer of experimentally verified manual GO annotation data to orthologs using Ensembl Compara
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Purification and characterization of beta-adrenergic receptor mRNA-binding proteins.
Stabilization of urokinase and urokinase receptor mRNAs by HuR is linked to its cytoplasmic accumulation induced by activated mitogen-activated protein kinase-activated protein kinase 2.
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HuR directly stabilizes an AU-rich-element RNA substrate and uPA/uPAR transcripts.
"HuR stabilized an ARE(uPA)-containing RNA substrate in vitro"
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MK2-dependent cytoplasmic HuR accumulation correlates with ARE-mRNA stabilization.
"enhance the cytoplasmic accumulation of endogenous HuR"
Facilitation of mRNA deadenylation and decay by the exosome-bound, DExH protein RHAU.
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HuR participates in RNA-dependent recognition of the uPA AU-rich element with RHAU.
"ARE(uPA)-mRNA recognition by RHAU may be mediated through its RNA-dependent interaction with the AUBPs HuR and NFAR1."
Involvement of KSRP in the post-transcriptional regulation of human iNOS expression-complex interplay of KSRP with TTP and HuR.
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HuR and KSRP compete for the same iNOS 3-prime-UTR AU-rich binding site.
"KSRP and HuR compete for this binding site"
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HuR functions as an iNOS mRNA-stabilizing protein.
"HuR, an iNOS mRNA stabilizing protein."
Identification and mechanistic characterization of low-molecular-weight inhibitors for HuR.
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HuR homodimerizes before RNA binding.
"HuR homodimerizes before RNA binding"
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Small molecules perturb HuR RNA binding and trafficking.
"These compounds interfere with HuR RNA binding, HuR trafficking"
Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells.
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Argonaute-containing mRNPs contain many RNA-metabolism proteins and were mapped by proteomics and co-immunoprecipitation.
"A comprehensive proteomic analysis of Ago-containing mRNPs identified a large number of proteins involved in RNA metabolism."
Novel tyrosine phosphorylated and cardiolipin-binding protein CLPABP functions as mitochondrial RNA granule.
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HuR is implicated in recruiting cytochrome-c transcript to a CLPABP RNA-protein granule.
"The CLPABP granule also contains the cytochrome c transcript, which may be mediated by the RNA-binding protein HuR."
Posttranslational modification of the AU-rich element binding protein HuR by protein kinase Cdelta elicits angiotensin II-induced stabilization and nuclear export of cyclooxygenase 2 mRNA.
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PRKCD-dependent phosphorylation promotes HuR nuclear export and COX-2 mRNA stabilization.
"increased nucleocytoplasmic HuR shuttling and depends on protein kinase Cdelta"
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HuR binds a distal AU-rich element in the COX-2 3-prime UTR.
"AngII-induced binding of HuR to this ARE"
Control of c-myc mRNA stability by IGF2BP1-associated cytoplasmic RNPs.
Ubiquitin-mediated proteolysis of HuR by heat shock.
Rapid and systematic analysis of the RNA recognition specificities of RNA-binding proteins.
Defining the membrane proteome of NK cells.
Raftlin is involved in the nucleocapture complex to induce poly(I:C)-mediated TLR3 activation.
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The article establishes Raftlin-dependent poly(I:C) uptake, but its searchable body does not expose an ELAVL1-specific assay.
"Raftlin is essential for poly(I:C) cellular uptake"
Coordinate regulation of GATA-3 and Th2 cytokine gene expression by the RNA-binding protein HuR.
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HuR binds the GATA3 3-prime UTR and promotes GATA3 mRNA stability and expression.
"HuR specifically interacts with its 3'UTR."
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HuR positively influences GATA3 mRNA turnover and Th2 cytokine expression.
"it positively influences GATA-3 mRNA turnover"
Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Angiogenesis inhibitor vasohibin-1 enhances stress resistance of endothelial cells via induction of SOD2 and SIRT1.
The structure of the ARE-binding domains of Hu antigen R (HuR) undergoes conformational changes during RNA binding.
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RRM1 is the primary HuR AU-rich-element-binding domain.
"RRM1 is the primary ARE-binding domain in HuR"
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RNA binding drives conformational changes and additional RRM2/linker contacts.
"conformational changes induce subsequent contacts of the RNA substrate with the inter-domain linker and RRM2"
A proteome-scale map of the human interactome network.
Quantitative interaction proteomics of neurodegenerative disease proteins.
Post-transcriptional Regulation of Nkx2-5 by RHAU in Heart Development.
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The study centers on RHAU-dependent Nkx2-5 mRNA turnover and translation in mouse heart development.
"The 3' UTR of Nkx2-5 mRNA possesses an AU-rich element (ARE) that facilitates RHAU-mediated mRNA decay."
ERK8 is a novel HuR kinase that regulates tumour suppressor PDCD4 through a miR-21 dependent mechanism.
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HuR binds the PDCD4 3-prime UTR and protects it from miR-21 silencing.
"HuR binds to the PDCD4 3'UTR to protect it from miR-21-induced silencing."
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ERK8 phosphorylation prevents HuR binding and permits miR-21-dependent PDCD4 decay.
"phosphorylation of HuR by ERK8 prevents it from binding to PDCD4 mRNA"
Antagonizing effect of CLPABP on the function of HuR as a regulator of ARE-containing leptin mRNA stability and the effect of its depletion on obesity in old male mouse.
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HuR stabilizes leptin ARE-containing mRNA, an effect antagonized by CLPABP.
"Increase in stability of ARE-containing mRNAs of leptin by HuR was antagonized by the expression of CLPABP"
IRAV (FLJ11286), an Interferon-Stimulated Gene with Antiviral Activity against Dengue Virus, Interacts with MOV10.
Role of N-myristoylation in stability and subcellular localization of the CLPABP protein.
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The CLPABP study describes ARE-mRNA stability control involving HuR.
"with the help of the RNA stabilizer, human antigen R (HuR)."
5-methylcytosine promotes pathogenesis of bladder cancer through stabilizing mRNAs.
Interactome Mapping Provides a Network of Neurodegenerative Disease Proteins and Uncovers Widespread Protein Aggregation in Affected Brains.
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The source is a large neurodegenerative-disease interaction network containing candidate interactions.
"connects ∼5,000 human proteins via ∼30,000 candidate interactions"
Cloning and characterization of HuR, a ubiquitously expressed Elav-like protein.
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Recombinant HuR avidly binds AU-rich elements in c-FOS and IL3 mRNAs.
"The purified recombinant protein binds avidly to the AU-rich element in c-fos and interleukin-3 mRNAs."
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All three AU motifs in the c-FOS core element are required for maximal binding.
"all three AU motifs are required for maximal binding."
HuR binds the CRM1 nuclear export machinery
HuR binds mRNAs in the nucleus
PKCdelta phosphorylates HuR
PKCalpha phosphorylates HuR
HuR:mRNA complex translocates from the nucleus to the cytosol
p-S158,221-HuR binds mRNA
p-S318,221-HuR binds mRNA
NS5 interacts with Spliceosome
UniProtKB record for human ELAVL1/HuR (Q15717)
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ELAVL1 is a reviewed 326-amino-acid human protein.
"Reviewed; 326 AA."
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ELAVL1 contains three annotated RNA-recognition-motif domains.
"FT DOMAIN 20..98"
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The reviewed record places ELAVL1 in both cytoplasm and nucleus.
"CC -!- SUBCELLULAR LOCATION: Cytoplasm"
Manual literature and annotation review notes for human ELAVL1
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All 93 grouped GOA annotations were manually adjudicated.
"All 93 grouped annotations were adjudicated."
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The review defines the core HuR mechanism and boundaries on broad propagated terms.
"## Core molecular mechanism"
Manual literature synthesis for human ELAVL1 (Q15717)
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ELAVL1 is a three-RRM nucleocytoplasmic shuttling RNA-binding protein.
"ELAVL1/HuR is a ubiquitously expressed, three-RRM, nucleocytoplasmic shuttling RNA-binding protein."
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Its defining activity is AU- and U-rich 3-prime-UTR recognition.
"Its defining molecular activity is recognition of AU- and U-rich elements in mRNA 3-prime UTRs."