Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by transferring manual GO annotations with predicted sequence features
Combined Automated Annotation using Multiple IEA Methods
Gene cluster for dissimilatory nitrite reductase (nir) from Pseudomonas aeruginosa: sequencing and identification of a locus for heme d1 biosynthesis.
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nirJ, in the nirSMCFDLGHJEN cluster, is necessary for heme d1 biosynthesis by insertion and complementation.
"Insertional mutation and complementation analyses showed that all of the nirFDLGHJE genes were necessary for the biosynthesis of heme d1."
NirJ, a radical SAM family member of the d1 heme biogenesis cluster.
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Purified NirJ has a 4Fe-4S centre and binds SAM, as a radical SAM enzyme.
"The purified protein was found to contain a 4Fe-4S centre, in line with the prediction that it belongs to the radical SAM class of enzymes."
The Radical SAM enzyme NirJ catalyzes the removal of two propionate side chains during heme d(1) biosynthesis.
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NirJ binds 12,18-didecarboxysiroheme and removes its two propionate side chains with SAM cleavage; keto groups are not introduced in vitro.
"we show that NirJ indeed catalyzes the removal of the two propionate side chains under simultaneous SAM cleavage."
The radical SAM enzyme NirJ cleaves off two propionate side chains with the release of acrylate during heme d(1) biosynthesis.
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Dinoroseobacter shibae NirJ has an auxiliary [4Fe-4S] cluster and releases acrylate; it is related to AhbC of the siroheme-dependent heme pathway.
"We show that acrylate is released during the NirJ reaction as the cleavage byproduct"