Gene Ontology annotation through association of InterPro records with GO terms
Use of the ND evidence code for Gene Ontology (GO) terms
Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
RNA polymerase II nuclear localization protein iwr1 (iwr1, SPAC23H4.08), Schizosaccharomyces pombe
Iwr1 directs RNA polymerase II nuclear import.
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In S. cerevisiae, RNA polymerase II nuclear import requires Iwr1, which binds Pol II in the active-center cleft between the two largest subunits, then uses an N-terminal bipartite NLS recognized by karyopherin-alpha to direct import; Iwr1 is subsequently displaced by initiation factors and recycled. The function is Pol II specific, transcription independent, and conserved from yeast to human.
"Here we show that Pol II nuclear import requires the protein Iwr1 and provide evidence for cyclic Iwr1 function. Iwr1 binds Pol II in the active center cleft between the two largest subunits, maybe facilitating or sensing complete Pol II assembly in the cytoplasm. Iwr1 then uses an N-terminal bipartite nuclear localization signal that is recognized by karyopherin alpha to direct Pol II nuclear import."
Iwr1 protein is important for preinitiation complex formation by all three nuclear RNA polymerases in Saccharomyces cerevisiae.
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Iwr1 was originally identified through its physical interaction with RNA polymerase II and is conserved throughout eukaryotes; in S. cerevisiae it is additionally important for preinitiation complex formation by all three nuclear RNA polymerases.
"Iwr1, a protein conserved throughout eukaryotes, was originally identified by its physical interaction with RNA polymerase (Pol) II."
Different pathways for the nuclear import of yeast RNA polymerase II.
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Budding-yeast Iwr1 facilitates import of assembled Pol II, but it is non-essential and parallel routes can import partial assemblies or individual subunits.
"However, as IWR1 is not an essential gene, Iwr1-independent pathway(s) for the nuclear import of Pol II must exist."
Iwr1 facilitates RNA polymerase II dynamics during transcription elongation.
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Budding-yeast Iwr1 has a direct nuclear role during Pol II elongation and enzyme disassembly from chromatin, in addition to its import function.
"Our results indicate that Iwr1 interacts with RNPII during elongation and is involved in the disassembly of the enzyme from chromatin."
Analysis of a genome-wide set of gene deletions in the fission yeast Schizosaccharomyces pombe.
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Genome-wide deletion analysis established a viable haploid deletion collection; PomBase associates iwr1 deletion viability with this screen.
"We found that 26.1% of fission yeast genes (1,260/4,836) were essential and 73.9% (3,576/4,836) were non-essential for viability of haploid cells in the growth conditions we used."
A systematic screen for morphological abnormalities during fission yeast sexual reproduction identifies a mechanism of actin aster formation for cell fusion.
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A systematic S. pombe deletion screen measured mating morphology; PomBase assigns decreased mating efficiency and multiseptate-cell phenotypes to iwr1 deletion.
"We report here on a systematic screen for morphological abnormalities of the mating process in fission yeast Schizosaccharomyces pombe."
Barcode sequencing and a high-throughput assay for chronological lifespan uncover ageing-associated genes in fission yeast.
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A barcoded deletion screen measured stationary-phase lifespan; PomBase assigns loss of stationary-phase viability to iwr1 deletion.
"We then applied a refined method of barcode sequencing (Bar-seq), addressing technical and statistical issues raised by persisting DNA in dead cells and sampling bottlenecks in aged cultures, to screen for mutants showing altered lifespan during stationary phase."
Broad functional profiling of fission yeast proteins using phenomics and machine learning.
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Broad chemical-genetic profiling measured deletion-mutant growth across many conditions; PomBase assigns hydroxyurea and methyl-methanesulfonate resistance to iwr1 deletion from this study.
"We assayed colony-growth phenotypes to measure the fitness of deletion mutants for 3509 non-essential genes in 131 conditions with different nutrients, drugs, and stresses."
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.