Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
UniProtKB/Swiss-Prot entry P22225 (PFR1_TRYBB)
The major component of the paraflagellar rod of Trypanosoma brucei is a helical protein that is encoded by two identical, tandemly linked genes.
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PFRA is a 600-aa highly helical protein encoded by two identical tandem genes.
"The PFR protein is coded for by two tandemly linked genes of identical nucleotide sequence."
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A single PFR protein was proposed to give both the 73 and 69 kDa bands via two conformations.
"This PFR protein can assume two different conformations, resulting in two distinct bands of apparent molecular masses of 73 and 69 kD in SDS-gel electrophoresis."
Paraflagellar rod is vital for trypanosome motility.
Flagellum ontogeny in trypanosomes studied via an inherited and regulated RNA interference system.
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Inducible PFRA RNAi blocks PFR construction and paralyses cells; PFR assembles distally into a stable structure.
"new PFRA RNA and PFRA protein quickly disappeared and PFR construction was affected, resulting in cell paralysis."
Calmodulin-binding properties of the paraflagellar rod complex from Trypanosoma brucei.
The paraflagellar rod of kinetoplastid parasites: from structure to components and function.
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PFR2 ablation is lethal in bloodstream forms in vitro and in mice.
"ablation of PFR2 is lethal in the mammalian bloodstream form both in vitro in culture and in vivo in mouse infections (Broadhead et al., 2006; Griffiths et al., 2007) (Fig. 3)."
A protein-protein interaction map of the Trypanosoma brucei paraflagellar rod.
Structure of the trypanosome paraflagellar rod and insights into non-planar motility of eukaryotic cells.
Calmodulin is required for paraflagellar rod assembly and flagellum-cell body attachment in trypanosomes.