azoR3 (PA3223, Q9HZ17) notes

Review session 2026-11-05 (PseudoCAP IBA-seed protocol, tier 2)

Fetched with just fetch-gene PSEAE Q9HZ17 --alias azoR3; accession matches the
worklist. Reviewed Swiss-Prot entry. No deep-research provider was used. Papers
come from the UniProt citations (PMID:24915188 full text; PMID:20417637 abstract
only); see genes/PSEAE/azoR1/azoR1-notes.md for the shared literature on the
three P. aeruginosa azoreductases. The PubMed MCP search returned API errors.

Literature

Purified paAzoR3 reduces quinones with NADH as donor
PMID:24915188,
preferring naphthoquinones
PMID:24915188,
and is the most active of the three (16.8 mM/s/mg with plumbagin)
PMID:24915188.
Distinct azo substrate profile among the paralogs
PMID:20417637.
No structure of paAzoR3 itself; FMN binding rests on HAMAP MF_01216 and the
homology model in PMID:20417637. No mutant phenotype reported.

Decisions

NEW: GO:0003955 NAD(P)H dehydrogenase (quinone) activity, IDA PMID:24915188 (core).
TOO_GENERAL: catalytic activity (IEA); GO:0016655 (IBA, PseudoCAP IDA, IEA).
ACCEPT: FMN binding (IEA).
KEEP_AS_NON_CORE: response to oxidative stress (IBA PTN000811013), as for azoR1.
MARK_AS_OVER_ANNOTATED: electron transfer activity (IEA).
REMOVE: GO:0016652 (UniRule mapping wrong; the UNIPROT defect is recorded once on
azoR1).
Counts: NEW 1, TOO_GENERAL 4, ACCEPT 1, KEEP_AS_NON_CORE 1,
MARK_AS_OVER_ANNOTATED 1, REMOVE 1.

PseudoCAP IBA-seed assessment

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN000024600	GO:0016655 oxidoreductase activity, acting on NAD(P)H, quinone or similar compound as acceptor	4	38 (25 taxa)	GO:0016655, IDA, PMID:24915188 (PseudoCAP)	SOUND	Full text read: purified paAzoR3 assayed with a quinone panel (NADH oxidation at 340 nm); highest rates of the three paralogs. Term correct; GO:0003955 added as NEW.	E. coli AzoR (P41407; EcoCyc IDA GO:0016655, PMID:19666717); azoR1 (Q9I5F3), azoR2 (Q9I2E2)	Yes; E. coli AzoR alone carries independent experimental evidence

No PSEUDOCAP and no GO_CENTRAL defect; node-level assessment shared with azoR1
(genes/PSEAE/azoR1/azoR1-notes.md), which also holds the UniRule GO:0016652
defect.