Human FXYD2 is the small gamma regulatory subunit of the sodium/potassium pump. Human expression and structural experiments support ATPase regulation and complex membership; ionic conditions alter the observed effects. This gives a more precise functional interpretation than the incoming channel-regulator labels while avoiding isolated gamma catalysis, universal activation/inhibition or magnesium-transporter claims.
All 25 original contexts are retained. The manual proposal has 10 ACCEPT, four KEEP_AS_NON_CORE, four TOO_GENERAL, three MODIFY and four UNDECIDED judgments. Actual PTHR14132 ancestry and both literal IBD donor sets are verified; the functional distinctions concern assay chemistry and unresolved conditional sign, not donor numbers or an invented sequence loss. The renal stress/growth and urinary-vesicle contexts remain non-core. The six original extension groups contain 16 AND-connected atoms and no OR alternatives.
The source guide gives public original URLs, selected locators and access limits. The evidence archive preserves original objects, the offline mapping, current natural-product sequences and all manual judgments. It also distinguishes historical construct/isoform names from current products. This dated entry records private proposal preparation, not genuine native fetch, validation or canonical authoring. Those later outcomes, if authorized, must be appended rather than retroactively inferred.
No new quotation is introduced at this stage. This is a scope choice, not a quotation prohibition: project policy permits concise, verified and claim-supporting source excerpts. Existing publication and family caches were not rewritten, and no provider-generated research file was created.
The repository tools produced 25 native annotation rows from the same 25 complete original contexts. Every one of the 16 TSV columns and all actual row positions were reconciled before transferring the approved judgments. The original six conjunctive extension groups were restored as 16 structured atoms through those actual joins. The two native product declarations are retained with the accepted sequence-scope descriptions.
All 28 planned normal source files are present: eight immutable existing files were reused and 20 were acquired. The native family slice matches both literal IBD assertions; original donor objects remain unchanged. The source guide separately records actual cache availability and the earlier selected-original reading limits. One short native PMID:33231612 Results excerpt now supports the core, with exact byte offsets and an original section locator in the evidence archive. This addition does not change any of the 25 annotation actions or the core biological scope. Validation, computed status and native history outcomes will be appended after execution.
Default schema, ontology, reference and best-practice validation passed with zero annotation advisories. Strict GOA reconciliation confirms all 25 annotations. The native status audit computed COMPLETE; the provisional status was updated to that exact value and checked again. The four source-specific unresolved contexts remain UNDECIDED. The genuine CREATE history and private rendered preview passed, including the source-guide and evidence-archive links. All 28 normal sources remain byte-identical to their acquisition record, and the single core excerpt remains an exact native-cache substring. No new annotation was added.
The pump-specific, sign-neutral process GO:1903406 replaces the broad GO:0043269 review decision, giving nine ACCEPT, four KEEP_AS_NON_CORE, four TOO_GENERAL, four MODIFY and four UNDECIDED annotations. The single regulatory-subunit core explicitly includes its already accepted sodium-export and potassium-import contributions within the complex. The two questioned sign-specific annotations remain unresolved: the human comparison gives FXYD1 and FXYD2 the same baseline Na-affinity direction, which does not establish loss of a phosphorylation-dependent donor phenotype, and the specific pig activation experiment remains unread. No new annotation, universal inhibitory activity or sequence-loss assertion is introduced.
The description now states the measured conditional Na-affinity direction and mouse pump-stability result. Rat gamma/alpha1 colocalization is located specifically in original Figure 2B/C, separate from the abstract's CHIF double-label result. Four additional short native-cache snippets anchor selected claims; the existing core snippet is preserved. Nine ambiguous optional full_text_unavailable fields are omitted while the exact cache metadata and external selected-reading scopes remain explicit. Native product identities are preserved without asserting functional isoform classes.
The evidence archive now retains each original context once, actual TSV/native locators, all six groups/16 atoms, product sequences, source access, selected reading scopes, PAINT joins and snippet offsets. Duplicate forecast/current scientific bodies and workflow status were removed; their complete first-publication artifact remains in Git at 89b0b04d9635104c1fb6a0b1689f0b512a0c1d12. Earlier dated entries describe that historical archive. This private follow-up has not yet been applied or validated; actual application and scoped outcomes will be recorded separately.
The approved review response was applied and passed default schema, ontology, reference and best-practice validation with zero annotation advisories. Strict GOA reconciliation preserves all 25 existing annotations, and the status audit confirms COMPLETE while all four UNDECIDED judgments remain. The private rendered preview contains 25 annotation rows, one core and 28 references, with working local source-guide, evidence-archive and notes links. All five quotation associations match their immutable native sources; all 28 source files remain unchanged. The genuine EDIT record links PR #5828; the original CREATE record is preserved. Native validation added only the official GO:1903406 row to the ontology cache; the targeted sorted/deduplicated-cache check passed. No new annotation was added.