Gene Ontology annotation through association of InterPro records with GO terms
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
Malonic semialdehyde reductase, succinic semialdehyde reductase, and succinyl-coenzyme A reductase from Metallosphaera sedula: enzymes of the autotrophic 3-hydroxypropionate/4-hydroxybutyrate cycle in Sulfolobales.
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Msed_1993 is the malonic semialdehyde reductase, identified by partial purification from autotrophic M. sedula and peptide mass fingerprinting.
"The best hit was with the gene gi|146304741 (100% identity, 54% coverage), annotated as 3-hydroxyacyl-CoA dehydrogenase (NAD binding) gene, encoding a 35.3-kDa protein (Msed_1993; 314 amino acids)."
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The recombinant enzyme is a homotrimer.
"The native molecular mass determined by gel filtration was 105 ± 10 kDa, suggesting a homotrimeric subunit composition."
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The enzyme prefers NADPH; NADH supports about 20% of the activity.
"NADH (20% activity) could partially substitute for NADPH (100%)."
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The enzyme is specific for malonate semialdehyde.
"Succinic semialdehyde, acetaldehyde, butyraldehyde, propionaldehyde, or glutaraldehyde did not serve as a substrate"
Exploiting microbial hyperthermophilicity to produce an industrial chemical, using hydrogen and carbon dioxide.
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Msed_1993 served as the malonate semialdehyde reductase (E3) of the CO2-fixing 3-hydroxypropionate module expressed in Pyrococcus furiosus.
"E3 (malonate semialdehyde reductase, Msed_1993)"
A 3-hydroxypropionate/4-hydroxybutyrate autotrophic carbon dioxide assimilation pathway in Archaea.
UniProt record A4YI81 (unreviewed)