ERG6 notes (P25087, YML008C)

• Function: SAM:Delta24-sterol C-methyltransferase, zymosterol -> fecosterol, EC 2.1.1.41 [UniProt:P25087]; PMID:6363386.
• Recombinant enzyme / AdoMet site: PMID:18563465.
• Genetics: erg6 null viable, no C-24 methylation PMID:2677674; pleiotropic membrane phenotypes.
• Location: mainly lipid droplets PMID:9450962; active in LD preps PMID:10515935; LD marker in PMID:24868093; ER pool enriched in PAM PMID:11298754.
• Mitochondrial signal: activity found in mito fraction but PMID:6363386; mito proteomics hits treated as contact-site co-purification -> MARK_AS_OVER_ANNOTATED.
• UniProt SubCell lacks lipid droplet (lists Microsome + Mitochondrion only).
• Self-interaction by split-ubiquitin, Y2H, DHFR-PCA -> identical protein binding KEEP_AS_NON_CORE.
• Decisions: 20 ACCEPT, 7 MODIFY, 6 MARK_AS_OVER_ANNOTATED, 3 KEEP_AS_NON_CORE.