Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
Sox10 regulates the development of neural crest-derived melanocytes in Xenopus.
-
Sox10 is expressed at the lateral neural plate edges at gastrulation; overexpression expands the Slug domain (C-terminus sufficient) and greatly increases pigment cells; competence lost during gastrulation.
"Overexpression of Sox10 causes a dramatic expansion of the Slug expression domain"
Sox10 is required for the early development of the prospective neural crest in Xenopus embryos.
-
Sox10 morpholino causes loss of NC precursors (slug, foxd3), increased apoptosis and decreased proliferation; Sox10 lies between Snail and Slug; also needed for melanocytes and ganglia.
"This effect of Sox10 depletion is produced during some of the earliest steps of neural crest specification"
SoxE factors function equivalently during neural crest and inner ear development and their activity is regulated by SUMOylation.
-
Sox9 and Sox10 are essential and functionally equivalent for NC precursor formation; SUMOylation directs SoxE activity to subsets of functions.
"Sox9 and the closely related factor Sox10 are essential for the formation of neural crest precursor cells"
A transition from SoxB1 to SoxE transcription factors is essential for progression from pluripotent blastula cells to neural crest cells.
-
Sox10 is not expressed in blastula pluripotent cells; it appears at the NPB by late gastrula/early neurula. Premature Sox10 represses pluripotency genes (HMG-dependent); Sox10 can replace SoxB1 for blastula pluripotency; Sox10 MO blocks NC markers in induced explants; SoxB1 cannot replace SoxE for NC.
"Sox9 and Sox10 do have the ability to maintain pluripotency, although they may do so less robustly than SoxB1 factors do"
SoxE gene duplication and development of the lamprey branchial skeleton: Insights into development and evolution of the neural crest.
-
Lamprey has three SoxE genes required for branchial skeleton; duplications occurred independently from gnathostome Sox8/9/10.
"understanding the independent evolution of duplicated SoxE genes among agnathan and gnathostome vertebrates"
Falcon deep research report on Xenopus laevis sox10
UniProtKB entry Q8AXX8 (SOX10_XENLA)