CHST6 (HGNC:6938; Q9GZX3) was authenticated as the next unchecked catalog gene after CHST3, with no ownership overlap in complete current-head PR file/label, alias/accession or queue checks. The genuine isolated normal intake produced 30 assertions and 12 original references, with no alternative-products field. All original machine objects, order, source terms, evidence, qualifiers, supporting entities and reference identities are preserved. GST-4alpha and GST-4beta in the older literature name separate human CHST5 and CHST6 genes, not alternative CHST6 splice products.
Actual Falcon timed out and Perplexity-lite returned HTTP 401 insufficient quota. Those failed attempts are retained; no provider-named report was fabricated. Manual primary-source review supplies the evidence.
PMID:11278593(https://doi.org/10.1074/jbc.M009995200), Methods construct/preparation sections and Results enzymatic activity, product analysis and coexpression, directly tests human corneal CHST6. Intact pcDNA3 and soluble catalytic-domain constructs were expressed in HeLa cells; the soluble cassette carries a secretion signal and polyhistidine sequence. Cell lysate or concentrated conditioned medium was used in PAPS-dependent assays. Mock controls, glycosidase cleavage and HPLC distinguish GlcNAc C6 sulfation from Gal sulfation. The separate hKSG6ST supplies Gal-6 modification; joint expression creates strongly 5D4-reactive keratan products. Mouse intestinal enzyme is a separate comparator. These are enzyme/product experiments, not disease necessity alone. Actual indexed author-upload Methods and Results were read; normal caches remain abstract-only. No figure pixels or exact modern sequence alignment was claimed.
PMID:17690104(https://doi.org/10.1074/jbc.M703695200), Methods and initial/sequential synthesis Results, reuses the human CGn6ST constructs from reference 25, the 2001 paper. Lec20 is the expression host, not the enzyme species. Concentrated soluble-enzyme mixtures with beta3GnT7 and beta4GalT4 produce extended GlcNAc-sulfated chains; subsequent Gal sulfation increases total sulfation. CHST6 performs transfer, while partner enzymes elongate the chain or sulfate Gal. The corneal-cell siRNA experiment targets the two glycosyltransferases, not CHST6. Selected full primary sections and reference 25 were read through authentic indexed author text; no whole-paper or figure-pixel read is claimed.
GO:0045130 is retained at its broader keratan-sulfotransferase scope. Although its current exact synonym is Gal-specific, official EC2.8.2.21 explicitly includes C6 sulfation of both Gal and GlcNAc. This is not evidence that CHST6 sulfates Gal. One precise GO:0001517 core describes GlcNAc-6 transfer, coupled to existing keratan-sulfate biosynthesis GO:0018146 and Golgi membrane location. No NEW process or molecular-function annotation is proposed.
PMID:11352640(https://pubmed.ncbi.nlm.nih.gov/11352640/) explicitly reports CHST6 sulfation of GlyCAM-1 in COS-7 coexpression. Its selected abstract is genuine; bounded full-body attempts failed. The positive assay is retained without inventing native leukocyte adhesion or immune trafficking. Source rows 15 and 18 are refined to GO:0051923 sulfation, which describes sulfate addition to a molecule. GO:0006477 protein sulfation instead requires a sulfate ester on a protein amino acid and would not describe the measured glycan chemistry. These rows are not recast as keratan assays.
PMID:11181564(https://pubmed.ncbi.nlm.nih.gov/11181564/) provides family/genomic context and an accessible sequence legend for human GST-4beta. Its family Golgi statement and the predicted type II anchor are distinct from direct target imaging. Existing compartment claims are retained at their evidence level. The TGN IBA includes actual CHST4/Q8NCG5 experimental-source context; the historical PTN002581838 placement was not reconstructed. Other PAINT nodes likewise remain historically unresolved despite positive target enzyme evidence. Donor counts and legitimate self-evidence are not weaknesses. Exact UniProt MGI crossreferences resolve mouse Chst4 and Chst5; unsuccessful initial id-query results are not biological negatives.
Obsolete GO:0006044 was retired as an unnecessary grouping with no official replacement. Its original machine IDs remain unchanged. Recommended terms use independently established performed chemistry, with source-specific mucin and keratan scopes explicit. The disease Reactome event describes loss of the normal reaction and does not claim every mutant retains catalysis. Its actual entity chain resolves to CHST6/Q9GZX3. The corneal genetic papers supply variant/expression context, not purified assays of all event variants.
All four original normal publication caches are genuine abstracts. Nine selected PMID caches remain abstract-only; external primary sections are separately recorded with exact URLs, locator ranges and receipt hashes. HTML access pages with misleading PDF filenames and metadata-only Elsevier responses are explicitly excluded as full text. One supplemental normal fetch (11042394) returned HTTP 429; four others succeeded. No retry was needed: the authentic source already published with CHST3 was selected.
Seven shared paths were authenticated at CHST3 PR4646 head dbe53dabacdf2df2a47e8016d68e6444c2b6177a: three PMID caches, two Reactome caches and two PANTHER family files. Exact published bytes were selected, while all original CHST6 normal archives and pre-selection variants remain untouched. This is shared-source applicability, not a claim that those paths already exist on main. Final main and open-PR collision checks determine publication preimages.
All primary quotations in the authored YAML, notes and artifact, including repeated anchors, are counted in the final audit. Notes and artifact add no primary quotations; full verbatim article receipts remain only in TMP, while the published artifact records URLs, response hashes, section/range locators and paraphrased scope. Bare file references link an authored provenance artifact and are not quoted as independent evidence. ROOT owns canonical files, history, Git and publication; this packet is TMP-only.