Q9VAS1 is the exact 671-residue Nepl19 product. PMID:34189422(https://pubmed.ncbi.nlm.nih.gov/34189422/) is cached with full text and classifies the expanded fly M13 family into catalytic Nep and motif-deficient Nepl proteins. Reproducible exact-sequence alignment compares human neprilysin P08473 and mouse positive control Q61391 using both L-INS-i and G-INS-i. The result is specific: H584/E585 become Q511/Q512; H588, E647 and D651 map to retained H515, E560 and D564. Thus the HExxH motif is disrupted without relying solely on the Nepl name.
The N-terminal signal peptide and lack of a mature membrane anchor support secretion. Existing plasma-membrane assignments are modified to extracellular region; both NOT peptidase assertions are accepted. Proteolysis participation remains unresolved because loss of intrinsic catalysis does not prove lack of regulatory involvement. No specific core molecular function is claimed.
The selected frozen UniProt record is retained in Nepl19-uniprot-source.json, with the complete original prediction metadata in Nepl19-predictions-source.json. It maps the exact accession to this FlyBase gene; current sequence identity does not establish the historical predictor input. Gene-level experiments support conserved functions unless a relevant isoform difference is established. Falcon research was requested with perplexity-lite fallback alongside publication caching. Primary publications and sequence observations, rather than AI summaries or ARBA assertions, support the curated conclusions.
The completed Falcon report was read in full. Its classification of Nepl19 as a predicted secreted inactive M13 homolog agrees with the independently inspected primary family study (PMID:34189422) and exact-sequence alignment. The report identifies spermathecal transcript detection as a possible experimental context; this is not evidence of a peptide substrate, reproductive function or catalytic activity and does not change the annotations. Its generic peptide-sequestration model remains an untested hypothesis. The decisive catalytic evidence remains the independently reproducible H584/E585-to-Q511/Q512 substitutions, not the provider verdict.