Deep research report on DVU_1606 TrkA-family potassium uptake regulatory protein
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DVU_1606 is a TrkA-family RCK regulatory subunit that assembles into an octameric gating ring
"DVU_1606 (TrkA-family RCK protein) is a cytosolic/peripheral protein that assembles into an octameric RCK gating ring associated with an inner-membrane K+ pore"
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ATP binding promotes active square-like conformation; ADP stabilizes inactive diamond-like state
"ATP binding to RCK_N intra-dimer interfaces stabilizes an activating square ring conformation; ADP stabilizes an inactivating diamond conformation"
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TrkA proteins are c-di-AMP receptors linking K+ homeostasis to signaling
"c-di-AMP binds many RCK/CBS-containing K+ transport regulators with affinities spanning ~40 nM to low µM"
Gene Ontology annotation through association of InterPro records with GO terms
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InterPro domains IPR003148 (RCK_N), IPR006037 (RCK_C) map to K+ transport
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Mapping is appropriate for BP but over-annotates MF as transporter activity
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping
Automatic assignment of GO terms using logical inference
Structural basis and synergism of ATP and Na+ activation in bacterial K+ uptake system KtrAB
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2.8 A cryo-EM structures of B. subtilis KtrAB
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KtrA forms octameric cytosolic RCK ring bound to dimeric KtrB pore
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ATP-bound adopts square-like active conformation
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ADP-bound adopts diamond-like inactive conformation
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Na+ binding at intra-dimer sites stabilizes active state
Bacterial cell volume regulation and the importance of cyclic di-AMP
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Comprehensive review of Trk/Ktr systems
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c-di-AMP binds RCK/CBS domains and reduces K+ import
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TrkA/KtrA are cytosolic regulatory subunits
TrkA of Streptococcus mitis CCUG31611 binds cyclic di-adenosine monophosphate and is required for growth in low potassium conditions
c-di-AMP accumulation impairs toxin expression of <i>Bacillus anthracis</i> by down-regulating potassium importers