Sources: UniProt Q14203, cached GOA-cited publications, plus PMID:25035494, PMID:25814576,
PMID:19874786, PMID:15173193, PMID:39115447 (all cached).
Key biology (with provenance):
- p150Glued is the dynein- and microtubule-binding shoulder projection of dynactin
PMID:25814576.
- Dynactin plus a cargo adaptor activates processive mammalian dynein
PMID:25035494.
- Neuron-specific anti-catastrophe / nucleation activity of the CAP-Gly+basic N-terminus
PMID:23874158.
- Nuclear envelope pool at prophase (Plk1 pS179) PMID:20679239.
- Nucleokinesis: p150 co-precipitates with Syne-1/Syne-2 in embryonic brain PMID:19874786;
nesprin-2 SR48-56 binds p150Glued PMID:39115447.
- Subdistal appendage pool recruited by KIF3A; MT anchoring and centriole cohesion PMID:23386061.
Decisions of note:
- No GOA annotation to dynactin complex (GO:0005869) or to a dynein-activating MF; proposed
MODIFY of microtubule associated complex / protein-containing complex rows to GO:0005869, and a
NEW contributes_to GO:0140660 cytoskeletal motor activator activity (PMID:25035494).
- Sterol sensor activity (contributes_to) REMOVED: ORP1L is the sensor, p150Glued the effector
(PMID:19564404 full text).
- NOT axonal transport (PMID:18364389) REMOVED: a negative result for one dominant allele does
not establish non-involvement; the G59S paper itself (PMID:16505168) proposes impaired
retrograde axonal transport.
- Organism-level phenotypes from transgenic mutant-p150 mice (motor behavior, neuromuscular
process, ventral spinal cord development, NMJ development) marked over-annotated.
- 43 of 44 protein binding rows REMOVED as uninformative; tau row MODIFIED to tau protein binding.
Deep research: DCTN1-deep-research-falcon.md arrived after the first pass; it agrees with the review (non-enzymatic dynein activator arm; retrograde axonal transport initiation; caution against assigning complex-level cargo functions to p150 alone). Its pointer to Singh 2024 LIS1-p150 was checked in PMID:38547289 and cited.