GO_REF:0000002

Gene Ontology annotation through association of InterPro records with GO terms

GO_REF:0000117

Electronic Gene Ontology annotations created by ARBA machine learning models

GO_REF:0000118

TreeGrafter-generated GO annotations

file:PSEPK/PP_3199/PP_3199-uniprot.txt

UniProtKB entry Q88I03 for Pseudomonas putida KT2440 PP_3199

The unreviewed target record identifies a monooxygenase with FAD-binding domains.

"InterPro; IPR050493; FAD-dep_Monooxygenase_BioMet."

PMID:27059806

Genetic and functional characterization of a novel meta-pathway for degradation of naringenin in Herbaspirillum seropedicae SmR1.

Disruption of Hsero_1007/fdeE prevented naringenin conversion, supporting an initiating monooxygenation step in the Herbaspirillum pathway.

"naringenin remained unaltered, suggesting that the FdeE protein is involved in the initial step of naringenin degradation."

PMID:39107441

Novel enzymatic route to the synthesis of C-8 hydroxyflavonoids including flavonols and isoflavones.

Purified Hsero_1007/FdeE is an FAD-dependent monooxygenase with broad activity across flavanones, flavones, isoflavones, and flavonols.

"the fdeE, the FMO from Herbaspirillum seropedicae SmR1 that is a part of the naringenin degradation pathway and is active towards a wide range of flavonoids-flavanones, flavones, isoflavones, and flavonols."

file:PSEPK/PP_3199/PP_3199-deep-research-openscientist.md

OpenScientist deep research report on PP_3199 (Q88I03)

The report identifies PP_3199 as an FdeE-like monooxygenase while noting that all direct enzymatic evidence comes from the Herbaspirillum ortholog.

"Direct KT2440 data are lacking:"

file:PSEPK/PP_3199/PP_3199-bioinformatics/RESULTS.md

Reproducible PP_3199 and Hsero_1007 FdeE sequence and reciprocal-hit comparison

Q88I03 and D8J0W9 are reciprocal proteome best hits and share 40.4% identity over 374 aligned residue pairs.

"Pairwise identity over aligned residue pairs: 40.4%"