aruH (PA4976, Q9HUI9) review notes
No deep-research API was available. Manual research on 2026-11-05 from the cached
publications (PMID:17416668 abstract; PMID:17416670 full text), a Europe PMC
title search ("aruH" / "arginine transaminase" AND Pseudomonas; PubMed MCP and
E-utilities were returning errors) and the UniProt record (reviewed).
Biology
- AruH is a class I PLP-dependent aminotransferase that catalyses the first step
of the arginine transaminase (ATA) pathway, L-arginine + pyruvate to
2-ketoarginine + L-alanine PMID:17416668. The recombinant enzyme was
purified to homogeneity; ping-pong kinetics, Km 13.9 mM for L-arginine and
1.6 mM for pyruvate, pH optimum 9.0, homodimer.
- Substrate range Arg > Lys > Met > Leu > Orn > Gln PMID:17416668.
- Genetics: an aruH knockout abolishes the arginine-inducible
arginine:pyruvate transaminase activity of an aruF background and its growth on
arginine PMID:17416670. The ATA pathway is the second aerobic
arginine catabolic route after the AST pathway; aruHI are induced by arginine
via AruRS.
- AruH is not the AspC aspartate transaminase it was originally annotated as
PMID:17416670.
- GO term: GO:0047635 L-alanine:oxo-acid transaminase activity has RHEA:13833
(L-arginine + pyruvate = 5-guanidino-2-oxopentanoate + L-alanine, EC 2.6.1.84)
as a narrowMatch xref, so it is the most specific existing GO term for the AruH
reaction (no EC 2.6.1.84-specific term exists in go-basic, checked
2026-11-05). GO:0047635 is_a GO:0140385 is_a GO:0008483, so the generic
transaminase rows are TOO_GENERAL.
PseudoCAP IBA-seed assessment
| PTN node |
node term |
n_seeds |
leaf IBAs |
seed annotation (term, evidence, PMID) |
verdict |
rationale |
other seeds |
IBD stands without this seed? |
| PTN002482828 |
GO:0008483 transaminase activity |
2 |
57 (41 taxa) |
GO:0008483, IDA, PMID:17416668 (PseudoCAP; UniProt has the same IDA) |
SOUND |
Purified recombinant AruH transaminates arginine with pyruvate; products identified by HPLC and MS; abstract states it outright. Under-specific (GO:0047635 fits better) but true. |
Synechocystis AspC Q55128 (EXP GO:0004069 aspartate transaminase, PMID:24302739, PMID:25070637) |
Yes |
The node mixes arginine:pyruvate and aspartate transaminases, so the generic
transaminase term is the right level. No PSEUDOCAP or GO_CENTRAL defect: the
PseudoCAP row is true, merely general, and UniProt independently made the same
IDA.
Action summary
ACCEPT: GO:0047635 (IEA), GO:0006527 (IDA, IMP, IEA). KEEP_AS_NON_CORE: GO:0030170
(IDA, IEA). TOO_GENERAL: GO:0003824, GO:0006520, GO:0008483 (IBA, IDA, IEA).