ppkA (PA0074, Q9I758) notes

Literature

No deep-research provider was available. PubMed MCP and E-utilities returned
errors on 2026-11-05, and PMC107787 (PMID:9852028) returned no body text. The
cached publications were searched for PpkA: PMID:9852028 (abstract),
PMID:17558395 (abstract), PMID:19400797 (full text), PMID:20114026 and
PMID:31208722 (context).

PpkA is a Hanks-type Ser/Thr kinase homolog, induced in infection and
required for full virulence PMID:9852028.
The seed paper's kinase evidence is an in vivo catalytic-site mutant
PMID:9852028.
PpkA phosphorylates Fha1 and triggers H1-T6SS; PppA antagonises
PMID:17558395.
In vitro kinase activity on Fha1 PMID:19400797.
Autophosphorylation sites Thr158/Thr161; dimerisation of the extracellular
domains activates; TagR acts upstream (PMID:19400797).

PseudoCAP IBA-seed assessment

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN001981567	GO:0004674 protein serine/threonine kinase activity	17	353 (73 taxa)	GO:0004674, IDA, PMID:9852028 (PseudoCAP)	INDIRECT-PHENOTYPE	The cited abstract infers kinase function from an in vivo catalytic-site mutant (overexpression toxicity), not from a kinase assay. The term is right: in vitro Fha1 phosphorylation and autophosphorylation are shown elsewhere (PMID:17558395, PMID:19400797). Row kept; evidence code questioned at SUSPECTED (full text not available), as for parS/parR.	M. tuberculosis PknA-L paralogs (P9WI81, P9WI79, P9WI67, P9WI65, P9WI75, P9WI77, P9WI83, P9WI71), B. subtilis PrkC O34507, human NEK6 Q9HC98, NEK7 Q8TDX7, MAP3K20 Q9NYL2, Plasmodium Q8I629, rat RGD:1311160, mouse MGI:1289156, worm WBGene00008956	Yes. Many seeds are directly assayed Ser/Thr kinases; the generic term suits a Hanks-kinase node. No GO_CENTRAL defect.

PSEUDOCAP defect: one WRONG_EVIDENCE_CODE, SUSPECTED. pppA (PA0075) is the
cognate phosphatase and is reviewed separately; it is not on this node. parS
and parR are two-component proteins on different nodes, so no consistency
issue arises.

Other decisions

GO:0050714 positive regulation of protein secretion (IDA): ACCEPT; no
T6SS-specific regulation term exists.
NEW GO:0005886 plasma membrane (IC): type II TM topology plus
periplasmic-dimerisation activation.
GO:0000166, GO:0004672, GO:0016301, GO:0016740: TOO_GENERAL.