Gene Ontology annotation through association of InterPro records with GO terms
Use of the ND evidence code for Gene Ontology (GO) terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Combined Automated Annotation using Multiple IEA Methods
The oligomeric stromal proteome of Arabidopsis thaliana chloroplasts.
Identification of the photorespiratory 2-phosphoglycolate phosphatase, PGLP1, in Arabidopsis.
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Recombinant PGLP1 has phosphoglycolate phosphatase activity; the pglp1-1 knockout loses 97% of leaf activity and requires elevated CO2; CS119 is a splice-site allele of At5g36700; PGLP2 (At5g47760) is active but not photorespiratory.
"These combined data establish At5g36700 as the gene encoding the photorespiratory PGLP, PGLP1."
Sorting signals, N-terminal modifications and abundance of the chloroplast proteome.
Quantitative proteomics of a chloroplast SRP54 sorting mutant and its genetic interactions with CLPC1 in Arabidopsis.
AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins.
Analysis of the Arabidopsis cytosolic proteome highlights subcellular partitioning of central plant metabolism.
Photorespiration.
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Review of Arabidopsis photorespiration; PGLP catalyses the entry reaction and At5g36700 encodes the photorespiratory PGLP.
"Thus, At5g36700 encodes the photorespiratory PGLP in Arabidopsis."
A proteomic strategy for global analysis of plant protein complexes.
Global Analysis of Membrane-associated Protein Oligomerization Using Protein Correlation Profiling.
The Photorespiratory Metabolite 2-Phosphoglycolate Regulates Photosynthesis and Starch Accumulation in Arabidopsis.
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Lines with altered PGLP1 activity show that 2-phosphoglycolate inhibits triose-phosphate isomerase and sedoheptulose-1,7-bisphosphatase, linking PGLP1 activity to Calvin-Benson cycle operation and starch allocation.
"2PG mechanistically achieves this by inhibiting the Calvin-Benson cycle enzymes triose-phosphate isomerase and sedoheptulose 1,7-bisphosphate phosphatase."
Hydrogen peroxide sulfenylates and inhibits the photorespiratory enzyme PGLP1 to modulate plant thermotolerance.
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Heat-induced chloroplast H2O2 sulfenylates PGLP1 (likely Cys-86) and inhibits it; PGLP1 levels affect thermotolerance via 2PG accumulation.
"H2O2 stimulates sulfenylation of the chloroplast-localized photorespiratory enzyme 2-phosphoglycolate phosphatase 1 (PGLP1) at cysteine 86, inhibiting its activity"