A genuine FOXJ1 fetch created the untouched 71-record seed and 72-row GOA export. All 18 qualified normal sources are now present. The reviewed science has been applied to the genuine seed. Validation and rendering outcomes will be appended after execution.
The official reviewed identity is FOXJ1 (HGNC:3816), UniProt Q92949, with a 421-aa sequence and no reported alternative-product or VAR_SEQ entries in the retained official response. Direct UniProt REST requests failed during intake; the official EBI Proteins response supplied the independently checked sequence and PTHR46805 family assignment. At genuine acquisition, current official UniProt JSON and text both succeeded and matched the same reviewed human identity and sequence.
The complete source capture contains 72 original QuickGO contexts. The genuine export contains 72 TSV records and the genuine seed contains 71 distinct records, exactly matching the private exporter projection and every seed machine field. Raw 55/56 are separate ISS cilium-assembly observations whose source identity, assigned database and date differ; both are embedded in the source evidence. Raw 34/62 retain their original occurs_in CL:0000706 extensions. The evidence now supplies actual raw-to-TSV-to-stored joins from the untouched seed.
The raw dispositions are 12 ACCEPT, 20 KEEP_AS_NON_CORE, 17 TOO_GENERAL, 4 MODIFY, 17 UNDECIDED and 2 MARK_AS_OVER_ANNOTATED. The genuine 71-record review totals differ only by one TOO_GENERAL because of the documented ISS collision. The review includes one nuclear Pol II transcriptional-activator core and 15 source assessments. No NEW annotations, quotations or natural-product assignments are added.
Human ciliary-gene induction and promoter activity support the activator core. Mouse DNA-selection/EMSA experiments supply conserved biochemical grounding and remain explicitly mouse. Original RFX2 interaction contexts are refined consistently using actual same-pair Y2H/AP-MS evidence; RFX3-dependent enhancement from the human functional paper is a separate observation. Source 25609649's exact pair remains unverified despite independent same-RFX2 support.
The peripheral immune evidence is retained within its measured scope. The two central B-cell-tolerance transfers are marked as over-annotated, while the branch-specific non-canonical NF-kappaB claims remain undecided. Expression, lineage tracing and downstream ciliopathy outcomes are kept distinct from direct developmental participation and protein perturbation.
The source guide gives public original links, named source sections and exact reading limits. The source evidence preserves all original objects, donor records, actual and original forecast joins, two extensions, phylogenetic grounding and the reviewed judgments. All input and output hashes are pinned in a private handoff; no private filesystem path is required to interpret these portable documents.
The 18 qualified normal paths comprise six existing-main publications reused unchanged and 12 genuine new source files. All protected sources from the completed prior review remain byte-exact. Fresh identity, all 72 original objects, all export columns and all 71 seed machine records matched the approved input. The native PAINT command produced four rows from 55 resolved nodes, with all four original IBD assertions retained. A genuine CREATE history records this session; validation/status and rendering remain pending. Falcon and Perplexity remain on the established credential hold; manual primary-source research is the current evidence route. No new provider attempt is planned unless the credential state actually changes; any later authorized attempt must use the explicitly existing client and record its actual outcome. A private primary retrieval is not a normal-cache acquisition.
Default gene validation, including ontology and markdown-reference checks, passed. Strict GOA matching passed for all 71 stored annotations. One advisory remains: the unrestricted epithelium-development IEA is TOO_GENERAL, while the original ISS context restricted to choroid plexus epithelial cells is UNDECIDED. The original extension and its source-specific uncertainty are retained. The repository computes DRAFT from this advisory; the 17 UNDECIDED judgments alone do not determine status. The initial status command reported the existing INITIALIZED field without overwriting it; only that computed status was corrected, and the subsequent audit passed.
The genuine CREATE history passed validation. Exact conservation confirmed all 72 original contexts, 71 source dictionaries and approved judgments, both CL extensions and both collision originals, one core, 22 references and 15 assessments, and all 18 normal source files. The prior accepted review's files, branch tips, scratch and Orca links remain intact. Private rendered HTML contains 71 annotation rows, one core and 22 references, with all 15 original-study links and the local source-guide/evidence links verified. The dependency deprecation notice is separate from the curation advisory. A private checker initially expected a field named review instead of the evidence's actual proposed_review; the corrected replay passed without changing source or scientific content. No provider job, new environment or broad corpus validation was run.
The nuclear transcriptional-activator core includes the existing accepted motile-cilium assembly and positive regulation of lung ciliated-cell differentiation terms. FOXJ1 increases ciliary-gene RNA and promoter activity in human airway basal cells (PMID:23822649(https://pubmed.ncbi.nlm.nih.gov/23822649/)); FOXJ1-variant human respiratory cells show impaired ciliogenesis and differentiation/flow (PMID:31630787(https://pubmed.ncbi.nlm.nih.gov/31630787/)). These distinct organelle-assembly and cell-differentiation scopes are retained within one transcriptional activity. Gene transfer alone is not treated as complete multiciliated differentiation.
Conserved sequence recognition is supported by mouse full-length HFH-4 and purified-domain assays (PMID:9096351(https://pubmed.ncbi.nlm.nih.gov/9096351/)). The original RFX2 interactions use source-specific human Y2H/AP-MS evidence (PMID:32296183(https://pubmed.ncbi.nlm.nih.gov/32296183/), PMID:28514442(https://pubmed.ncbi.nlm.nih.gov/28514442/), PMID:33961781(https://pubmed.ncbi.nlm.nih.gov/33961781/)); RFX3 enhancement of the human ciliary-gene program is separately supported by PMID:23822649. The exact original PMID:25609649 pair remains unverified.
Mouse B-cell restraint and the caution about breaking tolerance come from selected original publisher sections of PMID:16002694(https://pubmed.ncbi.nlm.nih.gov/16002694/). Apical-actin/docking and RhoA/B activation limits come from selected original publisher sections of PMID:17488776(https://pubmed.ncbi.nlm.nih.gov/17488776/). Both repository caches remain abstract-only; their accessible selected full-text sections justify removing the authored full_text_unavailable flags without changing any cache bytes or claiming complete supplementary/pixel inspection.
Clinical left/right randomization is a developmental patterning endpoint. Hydrocephalus can arise through altered cerebrospinal-fluid flow, so it does not alone resolve the brain-development or original choroid-plexus context. The existing laterality and brain actions remain unchanged. Both source observations at raw 55/56 retain explicit identical TOO_GENERAL judgments in the evidence; their provenance-only collapse remains one stored row. No new quotations or annotations are introduced. The published acquisition and validation history above is retained; follow-up check outcomes will be appended after authorized application.
The reviewed core and access clarifications passed default ontology/reference validation, strict GOA matching for all 71 stored annotations, the status audit and genuine EDIT-history validation. The computed status remains DRAFT with the same single GO:0060429 advisory distinguishing the unrestricted IEA from the original choroid-plexus cell context; no action or source field was changed to suppress it. Private rendering and link checks passed.
Exact conservation confirms all 72 originals, 71 source dictionaries and actions, both CL atoms, the duplicate provenance, 18 source files, and the prior histories and worktree state. Validation generated one ontology-cache row for GO:1901248; that generated change was preserved privately and the original cache bytes restored before the successful conservation check. The dependency deprecation notice is separate from the curation advisory. No provider call or broad corpus validation was run.