dsbB2 (PA0538, P57701) review notes

DsbB family (Pfam PF02600; IPR003752; PANTHER PTHR36570), four TM helices,
two periplasmic disulfides (UniProt FT DISULFID). Reviewed together with its
paralog dsbB1 (genes/PSEAE/dsbB1); both reviews make the same calls.

Naming caution

Arts et al. 2013 worked in PA14: PMID:24327342. UniProt PA14 entries Q02TM7 (PA14_07000) and Q02EA7
(PA14_69400) are identical in sequence to PAO1 P57701 (PA0538, UniProt
"dsbB2") and P21482 (PA5256, UniProt "dsbB1") respectively (full sequences
compared, 2026-11-05). So this gene (PA0538) is the paper's PaDsbB1 (PA14_07000). Both paralogs
carry identical PseudoCAP rows, so the swap causes no annotation error; it is
raised as a suggested question.

Literature (no deep-research provider; own reading, 2026-11-05)

Arts et al. 2013 (full text, PMC3870256): either DsbB keeps DsbA1 oxidised
PMID:24327342; in vitro reconstitution with decylubiquinone PMID:24327342; the double mutant loses pilus, flagellum and exoprotein folding
and virulence, complemented by either gene PMID:24327342.
E. coli DsbB (P0A6M2), the node's GO:0015035 seed: GO:0015035 IMP
PMID:8430071, GO:0016672 IDA PMID:10428033 (EcoCyc; QuickGO 2026-11-05).
Comparator for protein folding: E. coli DsbC (P0AEG6) carries GO:0006457 by
IDA, so periplasmic disulfide oxidoreductases do carry this process term.

PseudoCAP IBA-seed assessment

PTN node	node term	n_seeds	leaf IBAs	seed annotation (term, evidence, PMID)	verdict	rationale	other seeds	IBD stands without this seed?
PTN002195183	GO:0006457 protein folding	2	11 (8 taxa)	GO:0006457, IMP, PMID:24327342 (PseudoCAP)	SOUND	Full text read: double mutant fails to fold disulfide-containing envelope proteins, complemented by either gene; DsbB does a step of oxidative folding (DsbA reoxidation, shown in vitro).	the paralog (dsbB1 (genes/PSEAE/dsbB1)), same paper and same sound evidence	Yes. The other seed is equally sound; the node is a DsbB clade and the term suits it

No PSEUDOCAP or GO_CENTRAL defect. The node's GO:0015035 IBD rests on
E. coli DsbB and is also sound.

Other decisions

NEW GO:0016672 (sulfur donor, quinone acceptor) IDA PMID:24327342, the
precise DsbB reaction, as for E. coli DsbB; used as the core MF.
GO:0009055 electron transfer activity MODIFY to GO:0016672.
GO:0016020 membrane TOO_GENERAL (plasma membrane accepted).