Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
The plastidic bile acid transporter 5 is required for the biosynthesis of methionine-derived glucosinolates in Arabidopsis thaliana.
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N-terminal IPMI1 (At3g58990)-GFP localizes to plastids; gene is a HAG1/MYB28 target.
"GFP fused to N-terminal fragments of BAT5, IPMI1, IPMI2, and IPMDH1, as well as the plastidic triose phosphate/phosphate translocator, which was used as a positive control, all localized to plastids of cultured Arabidopsis cells"
Arabidopsis thaliana encodes a bacterial-type heterodimeric isopropylmalate isomerase involved in both Leu biosynthesis and the Met chain elongation pathway of glucosinolate formation.
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SSU2 and SSU3 function in Met chain elongation and appear redundant; ssu3-1 (Ws) has a very weak phenotype.
"Nevertheless the alterations in the glucosinolate composition observed in these mutants suggest that IPMI SSU2 and IPMI SSU3 function in Met chain elongation"
Functional specification of Arabidopsis isopropylmalate isomerases in glucosinolate and leucine biosynthesis.
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LeuD1 (SSU2) and LeuD2 (SSU3) act redundantly in aliphatic glucosinolate biosynthesis and form stromal IPMIs with LeuC.
"Reverse genetics and metabolite profiling showed that AtLeuD1 and AtLeuD2 function redundantly in aliphatic glucosinolate biosynthesis, but AtLeuD3 is not likely to be involved in this pathway."
A proteomic strategy for global analysis of plant protein complexes.
In Arabidopsis thaliana Substrate Recognition and Tissue- as Well as Plastid Type-Specific Expression Define the Roles of Distinct Small Subunits of Isopropylmalate Isomerase.
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SSU3:GFP localizes to chloroplast-sized plastids in vascular-associated cells; SRR determines substrate preference.
"Our study shows that the substrate recognition region that differs between IPMI SSU1 and the other two IMPI SSUs determines the substrate preference of IPMI."
The small subunit 1 of the Arabidopsis isopropylmalate isomerase is required for normal growth and development and the early stages of glucosinolate formation.
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ssu2 ssu3 double knockout lacks C7/C8 glucosinolates and does not accumulate IPM; SSU3 restores long-chain glucosinolates.
"our metabolite profiling of an ipmi ssu2-1/ipmi ssu3-1 double knockout mutant showed the absolute requirement of these two genes for the biosynthesis of C7 and C8 glucosinolates"
Protein complex formation in methionine chain-elongation and leucine biosynthesis.
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LeuD1 and LeuD2 are redundantly involved in Met chain elongation; no LeuC-LeuD Y2H interaction detected.
"LeuD1 and LeuD2 are redundantly involved in Met chain − elongation of GLS biosynthesis, whereas LeuD3 functions in Leu biosynthesis"
Biosynthesis of glucosinolates--gene discovery and beyond.