Gene Ontology annotation based on Enzyme Commission mapping
TreeGrafter-generated GO annotations
Combined Automated Annotation using Multiple IEA Methods
Hydrogen sulfide: a toxic gas produced by dissimilatory sulfate and sulfur reduction and consumed by microbial oxidation.
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Comprehensive review of dissimilatory sulfate reduction enzymology
"This review provides an examination of cytochromes, iron-sulfur proteins, and sirohemes participating in electron movement in diverse groups of sulfate-reducing, sulfur-reducing, and sulfide-oxidizing Bacteria and Archaea"
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Review of enzymatic activities in sulfate reduction
"biosulfur reduction or oxidation requires unique enzymatic activities with metal cofactors participating in electron transfer"
Proteomic and Isotopic Response of Desulfovibrio vulgaris to DsrC Perturbation.
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DvH proteomics maps AprA to DVU0847 with expression data
"The APS reductase subunit AprA (DVU0847) showed lower relative expression in the mutant"
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Shows coordinated regulation of AprA and QmoB
"The APS reductase subunit AprA (DVU0847) showed lower relative expression in the mutant (–0.35 ± 0.21), as did a subunit, QmoB (DVU0849), of its affiliated electron donor complex"
Large-scale genetic characterization of the model sulfate-reducing bacterium, Desulfovibrio vulgaris Hildenborough.
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RB-TnSeq study providing fitness data for DvH genes
"we generated a randomly barcoded transposon mutant library in the model SRB Desulfovibrio vulgaris Hildenborough (DvH) and used this genome-wide resource to assay the importance of its genes"
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Framework for understanding essentiality of sulfate reduction genes
"In addition to defining the essential gene set of DvH, we identified a conditional phenotype for 1,137 non-essential genes"
Deep research synthesis for aprA (Q72DT2)
Blinded OpenScientist function-assignment report (TreeGrafter audit)
AprA annotation re-review notes
Existing falcon AprA function-hypothesis report
Membrane protein complex of APS reductase and Qmo is present in Desulfovibrio vulgaris and Desulfovibrio alaskensis.
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AprA and AprB occur with Qmo in membrane preparations from D. vulgaris Hildenborough, with interaction corroborated by reciprocal affinity purification.
"another band contained three subunits of Qmo, as well as subunits of AprA and AprB."
OpenScientist AprA plasma-membrane localization hypothesis report
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The report identifies experimental evidence for a peripheral membrane-associated AprAB pool; this supports the broad compartment despite the report recommendation.
"There is genuine **experimental** evidence for a **peripheral, partially stable membrane-associated pool** of AprAB"