Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Electronic Gene Ontology annotations created by transferring manual GO annotations between related proteins based on shared sequence features
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Mechanism of transport of IFT particles in C. elegans cilia by the concerted action of kinesin-II and OSM-3 motors.
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Purified C. elegans kinesin-II is a heterotrimer of KLP-11, KLP-20 and KAP-1 (1:1:1, ~287 kD).
"consisting of 1 mol each of its subunits KLP-11, KLP-20, and KAP-1 with a native molecular mass of 287 kD"
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Kinesin-II is one of two anterograde IFT motors that move IFT particles along ciliary microtubules.
"the IFT particles assembling these sensory cilia are moved by the coordinate action of two anterograde IFT motors called kinesin-II and OSM-3, which are both members of the kinesin-2 family"
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Kinesin-II and OSM-3 act redundantly along the initial (middle) segment to build the cilium foundation.
"These motors function redundantly to move the same IFT particles along the initial segment and build the cilium foundation, with either motor but not both being dispensable for this function"
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Kinesin-II is a slow plus-end MT motor (~0.5 um/s) relative to OSM-3.
"kinesin-II alone moved MTs at a maximal rate of 0.3 μm/s, and OSM-3 alone moved them at 0.7 μm/s"
Regulation of a heterodimeric kinesin-2 through an unprocessive motor domain that is turned processive by its partner.
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KLP11/KLP20 is the C. elegans heterodimeric kinesin-2 studied here.
"KLP11/KLP20 of Caenorhabditis elegans cilia"
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Heterodimerization is required to bind KAP1, the motor-cargo link.
"heterodimerization is necessary to bind KAP1, the in vivo link between"