Gene Ontology annotation through association of InterPro records with GO terms.

Manual transfer of experimentally-verified manual GO annotation data to orthologs by curator judgment of sequence similarity.

Annotation inferences using phylogenetic trees

Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping

Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt.

Gene Ontology annotation based on curation of immunofluorescence data

Electronic Gene Ontology annotations created by ARBA machine learning models

Combined Automated Annotation using Multiple IEA Methods.

Novel dimerization fold of RAP30/RAP74 in human TFIIF at 1.7 A resolution.

Interaction with general transcription factor IIF (TFIIF) is required for the suppression of activated transcription by RPB5-mediating protein (RMP).

Transcription factors IIF and IIS and nucleoside triphosphate substrates as dynamic probes of the human RNA polymerase II mechanism.

Dynamic proteomics in individual human cells uncovers widespread cell-cycle dependence of nuclear proteins.

A comprehensive resource of interacting protein regions for refining human transcription factor networks.

Human-chromatin-related protein interactions identify a demethylase complex required for chromosome segregation.

Maximizing binary interactome mapping with a minimal number of assays.

A reference map of the human binary protein interactome.

AI-guided pipeline for protein-protein interaction drug discovery identifies a SARS-CoV-2 inhibitor.

Interaction with RAP74 subunit of TFIIF is required for transcriptional activation by serum response factor.

Roles for both the RAP30 and RAP74 subunits of transcription factor IIF in transcription initiation and elongation by RNA polymerase II.

Multiple functional domains of human transcription factor IIB: distinct interactions with two general transcription factors and RNA polymerase II.

RNA polymerase II-associated protein (RAP) 74 binds transcription factor (TF) IIB and blocks TFIIB-RAP30 binding.

Oestrogen receptor facilitates the formation of preinitiation complex assembly: involvement of the general transcription factor TFIIB.

Recruitment of RNA Polymerase II Holoenzyme by TFIIF to the pol II promoter:TFIID:TFIIA:TFIIB complex

Formation of the closed pre-initiation complex

Addition of nucleotides between position +11 and +30

Recruitment of elongation factors to form elongation complex

Hyperphosphorylation (Ser2) of RNA Pol II CTD by P-TEFb complex

Hypophosphorylation of RNA Pol II CTD by FCP1P protein

Addition of nucleotides leads to transcript elongation

Resumption of elongation after recovery from pausing

Abortive termination of elongation after arrest

Separation of elongating transcript from template

Formation of DSIF:NELF:early elongation complex

DSIF complex binds to RNA Pol II (hypophosphorylated)

Abortive termination of early transcription elongation by DSIF:NELF

2-4 nt.backtracking of Pol II complex on the template leading to elongation pausing

Pol II elongation complex moves on the template as transcript elongates

TFIIS-mediated recovery of elongation from arrest

7-14 nt. Backtracking of Pol II complex on the template leading to elongation arrest

Elongating transcript encounters a lesion in the template

Extrusion of 5'-end of 30 nt long transcript through the pore in Pol II complex

Phosphorylation of DSIF by the P-TEFb(Cyclin T1:Cdk9) complex

Phosphorylation of NEFL by the P-TEFb(Cyclin T1:Cdk9) complex

CDK12 phosphorylates RNA Pol II CTD at DNA repair genes

CCNK:CDK12 binds RNA Pol II at DNA repair genes

PTB and hnRNPA1 bind FGFR2 pre-mRNA to repress IIIb splicing and promote formation of FGFR2c mRNA

ESRP1 and 2 bind FGFR2 pre-mRNA to promote FGFR2b maturation and expression

FGFR2b-specific alternative splicing produces FGFR2b transcript

FGFR2c-specific alternative splicing produces FGFR2c transcript

CDK7 phosphorylates serine-5 and serine-7 of heptad repeats in C-terminal domain of RNA polymerase II at snRNA promoter

General transcription factors bind SNAPc:POU2F1:ZNF143:snRNA gene

RPAP2 binds RNA polymerase II phosphorylated at serine-7 residues of heptad repeats in the C-terminal domain

RNA polymerase II binds initiation factors at promoter of snRNA gene (U1, U2, U4, U4atac, U5, U11, U12)

Pre-snRNA transcript initiation, Integrator binding, LEC binding

Integrator complex processes the 3' end of snRNA

Pre-snRNA is elongated and capped with 7-methylguanosine

CBCAP complex binds 7-methylguanosine cap of snRNA

Internal Methylation of mRNA

Formation of pre-mRNPs

Abortive initiation after formation of the first phosphodiester bond

Formation of AT-AC C complex

Formation of AT-AC A complex

Formation of AT-AC B Complex

ATAC spliceosome mediated Lariat formation,5' splice site cleavage

ATAC spliceosome mediated 3' splice site cleavage, exon ligation

Binding of TFIIE to the growing preinitiation complex

Addition of the third nucleotide on the nascent transcript

Abortive Initiation Before Second Transition

NTP Binds Active Site of RNA Polymerase II

Fall Back to Closed Pre-initiation Complex

Newly Formed Phosphodiester Bond Stabilized and PPi Released

Nucleophillic Attack by 3'-hydroxyl Oxygen of nascent transcript on the Alpha Phosphate of NTP

Addition of the fourth nucleotide on the Nascent Transcript: Second Transition

Addition of Nucleotides 5 through 9 on the growing Transcript

Abortive Initiation After Second Transition

RNA Polymerase II Promoter Opening: First Transition

Addition of nucleotides 10 and 11 on the growing transcript: Third Transition

Activation of GT

RNA Polymerase II CTD (phosphorylated) binds to CE

Phosphorylation (Ser5) of RNA pol II CTD

SPT5 subunit of Pol II binds the RNA triphosphatase (RTP)

Capping complex formation

Hydrolysis of the 5'-end of the nascent transcript by the capping enzyme

Formation of the CE:GMP intermediate complex

Transfer of GMP from the capping enzyme GT site to 5'-end of mRNA

Dissociation of transcript with 5'-GMP from GT

Methylation of GMP-cap by RNA Methyltransferase

Recognition and binding of the mRNA cap by the cap-binding complex

ESR1:ESTG:P-TEFb recruited to paused RNA polymerase II on MYB gene

p-TEFb phosphorylates serine 2 in RNA polymerase II CTD

Unwinding of DNA for the Nascent Transcript: Second Transition

Unwinding DNA for the nascent transcript

Formation of the Spliceosomal E complex

Formation of the Spliceosomal A complex

Formation of the Spliceosomal B* complex

Formation of the Spliceosomal Pre-B complex

Formation of the Spliceosomal C* complex

Formation of the Spliceosomal B complex

Formation of the Spliceosomal Bact complex

Formation of the Spliceosomal P complex and exon ligation

Spliceosomal P complex dissociates yielding the intron-containing complex (ILS) and the spliced mRNP (new)

Formation of the Spliceosomal C complex containing intron lariat

Deep research report on GTF2F2