Compact archived record and sequence evidence for Q945B5
Electronic Gene Ontology annotations created by ARBA machine learning models
Gene duplication in the diversification of secondary metabolism: tandem 2-oxoglutarate-dependent dioxygenases control glucosinolate biosynthesis in Arabidopsis.
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Identifies AOP2 and AOP3 as tandem 2-ODDs at the GS-AOP locus; AOP2 expressed in E. coli converts 3-methylsulfinylpropyl and 4-methylsulfinylbutyl glucosinolates to allyl and 3-butenyl glucosinolates; Col-0 carries a frameshifted non-functional AOP2; no ecotype co-expresses AOP2 and AOP3.
"we show that AOP2 catalyzes the conversion of methylsulfinylalkyl glucosinolates to alkenyl glucosinolates"
The characterisation of AOP2: a gene associated with the biosynthesis of aliphatic alkenyl glucosinolates in Arabidopsis thaliana.
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Defines three AOP2 alleles (AOP2-1 Cvi/St, AOP2-2 Pi, AOP2-3 Col/Ler with 5-bp frameshift); AOP2 is light-regulated and most expressed in leaf and stem; overexpression of AOP2-2 in Col-0 converts methylsulfinylalkyl glucosinolates to 2-propenyl and 3-butenyl glucosinolates in planta.
"The over-expression of a functional AOP2 allele was found to successfully convert the precursor methylsulfinyl alkyl glucosinolate into the alkenyl form."
The Glucosinolate Biosynthetic Gene AOP2 Mediates Feed-back Regulation of Jasmonic Acid Signaling in Arabidopsis.
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AOP2 participates in a feedback loop linking glucosinolate biosynthesis to JA signaling, partly independent of MYB28/MYB29; mechanism unresolved, so no GO process term is proposed.
"Transcriptome analysis of these mutants identified a so far unrecognized link between AOP2 and jasmonic acid (JA) signaling independent of MYB28 and MYB29."
Natural variation in cross-talk between glucosinolates and onset of flowering in Arabidopsis.
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AOP2 introduced into AOP-null accessions alters glucosinolate levels and, depending on genetic background, flowering time.
"the effect on flowering time was dependent on the genetic background pointing to natural variation in cross-talk between defense chemistry and onset of flowering"
Biosynthesis of glucosinolates--gene discovery and beyond.
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Review placing AOP2 in the side-chain decoration phase of glucosinolate biosynthesis.
"Recent progress includes genes in all three phases of the pathway, i.e. side-chain elongation of precursor amino acids, formation of the core glucosinolate structure and side-chain decoration."