Normal-cache availability and independent original reading are recorded separately. Genuine native preparation completed the 22 missing records and preserved 17 inherited records. Titles and availability flags now match the normal caches; independent source-access limits remain unchanged.
Current IntAct records retain pooling, array and validated yeast two-hybrid evidence for human GSDME and TIFA. The historical dataset labels the GSDME construct O60443-1/ORF53167; present records use O60443. No independent clone-sequence equivalence or physiological complex is asserted.
The original study combines human familial segregation and splice analysis with mouse cochlear expression. The mutant frameshift product is distinct from current official splice products and caspase-generated fragments.
The published construct, MTT, colony and siRNA methods were inspected. Growth assays are context-specific; changes in AKT or cyclins do not identify a direct molecular target.
Official immunofluorescence annotations were inspected; image pixels were not reanalyzed.
Replayed PTHR15207 originals contain 59 nodes and 31 leaves. AN3/PTN002689666 lies on the target lineage; all three current IBD donor sets match. Older cached IBD nodes remain preserved. A separate pejvakin branch also descends from AN3, so this audit supports the target annotations without asserting uniform function across that clade.
The protein entities resolve to O60443 coordinates 1–496, 1–270 and 271–496; cleavage activity belongs to CASP3.
The exact NT entity retains its cytosolic compartment inside the separately localized membrane complex.
Repeated component edges are the curated reaction representation; they do not establish native pore stoichiometry.
GZMB is the catalytic entity; the GSDME substrate/products do not acquire a protease function.
The protein output is distinct from the nuclear gene and its regulators; no transcription-regulator activity is inferred for GSDME.
The final summary sentence says 1–275, but the linked protein explicitly spans 1–270. Both source statements are retained without coordinate correction.
Eleven members and four candidates trace to O60443, including truncations 1–209 and 1–45. Variant binding remains unresolved; no NOT assertion is transferred.
The separate source-evidence record contains original-source URLs, hashes and inspected section locators. It is independent of the raw GOA archive. The precursor, cleavage products, official splice products and pathogenic variants retain separate identities.
| Source | Original sections | Public record |
|---|---|---|
| PMID:26236191 | Methods; Results; Figures 3–4 | PMC4504148 |
| PMID:30976076 | Methods; Results; Figures 4–6 | PMC6459836 |
The source-evidence record gives exact captured-source hashes and selected BioC passage indices. Normal-cache availability flags remain unchanged.