HEM15 (YOR176W, P16622) notes

Ferrochelatase, terminal enzyme of heme biosynthesis.

• Activity: inserts Fe2+ into protoporphyrin IX PMID:2185242; reaction heme b + 2 H+ = protoporphyrin IX + Fe2+, EC 4.98.1.1 [UniProt:P16622].
• Purified recombinant (baculovirus) enzyme active, Km for mesoporphyrin and iron as for native PMID:1304908.
• Location: matrix side of inner mitochondrial membrane PMID:7629135; mislocalised (no presequence) enzyme still makes some heme but less, and b-type cytochromes drop 80% PMID:7629135.
• Genetics: hem15 ferrochelatase-deficient mutants PMID:7035824; gene cloned by complementation, 31-aa presequence PMID:2185242.
• Curation: YeastPathways RCA "cytosol" is a GO-CAM conversion default and is wrong for this mitochondrial enzyme -> REMOVE.