ACTL7B bioinformatics audit

Generated by analyze.py (propagation, residues, report). Regenerate with uv run --project . python analyze.py all from this directory; fetch timestamps are in results/*.json so this file is byte-reproducible.

1. Where every ACTL7B annotation comes from

All 11 rows of ACTL7B-goa.tsv and all 18 WITH/FROM tokens were parsed from the TSV and resolved. For every token that resolves to a protein, QuickGO was asked what evidence that protein carries for the term being propagated (term plus is_a/part_of descendants).

GOA row term code WITH/FROM token resolves to source's evidence for this term note
1 GO:0005634 nucleus IBA CGD:CAL0000196900 ARP9 (Candida albicans) Q5A9X7 [TrEMBL] IBA,IDA,IEA (own experimental: yes)
1 GO:0005634 nucleus IBA MGI:MGI:1343051 Actl7a (Mus musculus) Q9QY84 [Swiss-Prot] EXP,IBA,IDA,IEA (own experimental: yes)
1 GO:0005634 nucleus IBA PANTHER:PTN008986520 - - internal PANTHER tree node, not a protein
1 GO:0005634 nucleus IBA SGD:S000004636 ARP9 (Saccharomyces cerevisiae) Q05123 [Swiss-Prot] IBA,IDA,IEA,IPI (own experimental: yes)
1 GO:0005634 nucleus IBA UniProtKB:Q57ZL0 ? (Trypanosoma brucei brucei) Q57ZL0 [TrEMBL] HTP,IBA,IDA (own experimental: yes)
2 GO:0005737 cytoplasm IBA MGI:MGI:1343051 Actl7a (Mus musculus) Q9QY84 [Swiss-Prot] EXP,IBA,IDA,IEA,IMP,ISO,ISS (own experimental: yes)
2 GO:0005737 cytoplasm IBA MGI:MGI:1343053 Actl7b (Mus musculus) Q9QY83 [Swiss-Prot] IBA,IDA (own experimental: yes)
2 GO:0005737 cytoplasm IBA PANTHER:PTN001377938 - - internal PANTHER tree node, not a protein
2 GO:0005737 cytoplasm IBA RGD:1304697 Actl7a (Rattus norvegicus) Q641W9 [Swiss-Prot] EXP,IBA,IEA,ISO,ISS (own experimental: yes)
2 GO:0005737 cytoplasm IBA UniProtKB:Q9Y615 ACTL7A (Homo sapiens) Q9Y615 [Swiss-Prot] IBA,IDA,IEA,IMP,ISS (own experimental: yes)
3 GO:0005198 structural molecule activity IBA PANTHER:PTN000940351 - - internal PANTHER tree node, not a protein
3 GO:0005198 structural molecule activity IBA PANTHER:PTN008986528 - - internal PANTHER tree node, not a protein
4 GO:0005856 cytoskeleton IEA UniProtKB-SubCell:SL-0090 - - UniProt controlled-vocabulary subcellular-location term, not a protein
5 GO:0007010 cytoskeleton organization IEA GO:0005200 - - inter-ontology logical inference from another GO term, not a protein
6 GO:0005515 protein binding IPI UniProtKB:O00291 HIP1 (Homo sapiens) O00291 [Swiss-Prot] IBA,IDA,IEA,IPI,TAS (own experimental: yes)
7 GO:0005515 protein binding IPI UniProtKB:P13473-2 LAMP2 (Homo sapiens) P13473 [Swiss-Prot] IEA,IPI (own experimental: yes) isoform-level accession P13473-2
8 GO:0005515 protein binding IPI UniProtKB:P55212 CASP6 (Homo sapiens) P55212 [Swiss-Prot] IPI (own experimental: yes)
9 GO:0005515 protein binding IPI UniProtKB:P62826 RAN (Homo sapiens) P62826 [Swiss-Prot] IPI (own experimental: yes)

Ancestral PANTHER nodes cited by the IBA rows

Read from the cached PAINT table interpro/panther/PTHR11937/PTHR11937-paint.tsv. IRD rows with negated=true are PAINT's own explicit rejections of a term for that clade.

node term aspect PAINT evidence seeds
PTN000940351 GO:0005200 F IBD ACT1 (Saccharomyces cerevisiae), ACTR2 (Homo sapiens), Actg1 (Mus musculus), act1 (Dictyostelium discoideum), ARP10 (Saccharomyces cerevisiae), ACTR3 (Homo sapiens), act10 (Dictyostelium discoideum), Actg1 (Rattus norvegicus), ACTB (Homo sapiens), ARP1 (Saccharomyces cerevisiae)
PTN001377938 GO:0005737 C IBD Actl7a (Mus musculus), ACTL7A (Homo sapiens), Actl7a (Rattus norvegicus), Actl7b (Mus musculus)
PTN008986520 GO:0005634 C IBD Actl7a (Mus musculus), ARP9 (Saccharomyces cerevisiae), ? (Trypanosoma brucei brucei), ARP9 (Candida albicans)
PTN008986528 GO:0005198 F IBA PANTHER:PTN000940351
PTN008986528 GO:0005200 F IRD (NEGATED) PANTHER:PTN000940351

2. Does ACTL7B retain actin's nucleotide and polymerisation residues?

Residue sets are computed from deposited structures, then scored across an actin-family panel by global BLOSUM62 alignment. Contact cutoffs: 4.0 A to nucleotide/ion heavy atoms, 4.5 A between protomers.

Panel

accession gene UniProt name status length role in this panel % id to ACTL7B
Q9Y614 ACTL7B (Homo sapiens) Actin-like protein 7B Swiss-Prot 415 query (human ACTL7B) 100.0
Q9QY83 Actl7b (Mus musculus) Actin-like protein 7B Swiss-Prot 418 ACTL7 clade: mouse ortholog 86.7
Q9Y615 ACTL7A (Homo sapiens) Actin-like protein 7A Swiss-Prot 435 ACTL7 clade: human paralog 58.6
Q9QY84 Actl7a (Mus musculus) Actin-like protein 7A Swiss-Prot 440 ACTL7 clade: mouse paralog 58.1
P60709 ACTB (Homo sapiens) Actin, cytoplasmic 1 Swiss-Prot 375 conventional actin 44.3
P68133 ACTA1 (Homo sapiens) Actin, alpha skeletal muscle Swiss-Prot 377 conventional actin 42.4
P60010 ACT1 (Saccharomyces cerevisiae) Actin Swiss-Prot 375 conventional actin (yeast) 43.2
P61160 ACTR2 (Homo sapiens) Actin-related protein 2 Swiss-Prot 394 cytoplasmic ARP (Arp2/3 complex) 35.3
P61158 ACTR3 (Homo sapiens) Actin-related protein 3 Swiss-Prot 418 cytoplasmic ARP (Arp2/3 complex) 29.6
O96019 ACTL6A (Homo sapiens) Actin-like protein 6A Swiss-Prot 429 nuclear ARP (SWI/SNF-family) 29.6
Q9H9F9 ACTR5 (Homo sapiens) Actin-related protein 5 Swiss-Prot 607 nuclear ARP (INO80 complex) 31.1
Q9H981 ACTR8 (Homo sapiens) Actin-related protein 8 Swiss-Prot 624 nuclear ARP (INO80 complex) 25.1
Q05123 ARP9 (Saccharomyces cerevisiae) Actin-like protein ARP9 Swiss-Prot 467 nuclear ARP (yeast RSC/SWI-SNF) 20.2
Q12406 ARP7 (Saccharomyces cerevisiae) Actin-related protein 7 Swiss-Prot 477 nuclear ARP (yeast RSC/SWI-SNF) 24.1

1ATN - G-actin monomer with ATP (X-ray)

Actin chains detected: A; chain analysed: A (373 SEQRES residues). Nucleotide/ion groups: ATP, CA, MG.

nucleotide contacts - 22 positions: [14, 15, 16, 17, 19, 75, 138, 156, 157, 158, 159, 160, 183, 184, 211, 214, 215, 302, 303, 304, 306, 307]

protein role % id to whole structure seq identical similar different gap % identical % id+sim
ACTL7B (Homo sapiens) query (human ACTL7B) 42.6 14 1 7 0 63.6 68.2
Actl7b (Mus musculus) ACTL7 clade: mouse ortholog 43.2 14 1 7 0 63.6 68.2
ACTL7A (Homo sapiens) ACTL7 clade: human paralog 42.9 13 3 6 0 59.1 72.7
Actl7a (Mus musculus) ACTL7 clade: mouse paralog 43.2 13 2 7 0 59.1 68.2
ACTB (Homo sapiens) conventional actin 93.3 21 1 0 0 95.5 100.0
ACTA1 (Homo sapiens) conventional actin 99.7 22 0 0 0 100.0 100.0
ACT1 (Saccharomyces cerevisiae) conventional actin (yeast) 86.6 20 2 0 0 90.9 100.0
ACTR2 (Homo sapiens) cytoplasmic ARP (Arp2/3 complex) 48.8 19 2 1 0 86.4 95.5
ACTR3 (Homo sapiens) cytoplasmic ARP (Arp2/3 complex) 40.8 17 4 1 0 77.3 95.5
ACTL6A (Homo sapiens) nuclear ARP (SWI/SNF-family) 40.2 11 2 9 0 50.0 59.1
ACTR5 (Homo sapiens) nuclear ARP (INO80 complex) 33.2 9 1 12 0 40.9 45.5
ACTR8 (Homo sapiens) nuclear ARP (INO80 complex) 24.4 11 4 7 0 50.0 68.2
ARP9 (Saccharomyces cerevisiae) nuclear ARP (yeast RSC/SWI-SNF) 23.6 7 4 11 0 31.8 50.0
ARP7 (Saccharomyces cerevisiae) nuclear ARP (yeast RSC/SWI-SNF) 25.7 7 4 11 0 31.8 50.0

Per-position nucleotide contacts in ACTL7B (1ATN numbering -> ACTL7B): G14->G57, S15->S58, G16->Q59, L17->Y60, K19->K62, G75->G118, Q138->Q181, S156->S199, G157->G200, D158->H201, G159->G202, V160->V203, G183->G226, R184->G227, R211->E253, K214->K256, E215->K257, G302->G343, G303->G344, T304->C345, M306->M347, Y307->L348

8A2S - F-actin filament, Mg-ADP-Pi state (cryo-EM)

Actin chains detected: C, A, B, D, E; chain analysed: C (375 SEQRES residues). Nucleotide/ion groups: ADP, PO4, MG.

nucleotide contacts - 20 positions: [13, 14, 15, 16, 18, 137, 156, 157, 158, 159, 182, 210, 213, 214, 301, 302, 303, 305, 306, 336]

protein role % id to whole structure seq identical similar different gap % identical % id+sim
ACTL7B (Homo sapiens) query (human ACTL7B) 42.9 13 1 6 0 65.0 70.0
Actl7b (Mus musculus) ACTL7 clade: mouse ortholog 43.5 13 1 6 0 65.0 70.0
ACTL7A (Homo sapiens) ACTL7 clade: human paralog 43.2 12 3 5 0 60.0 75.0
Actl7a (Mus musculus) ACTL7 clade: mouse paralog 43.7 12 2 6 0 60.0 70.0
ACTB (Homo sapiens) conventional actin 93.3 19 1 0 0 95.0 100.0
ACTA1 (Homo sapiens) conventional actin 100.0 20 0 0 0 100.0 100.0
ACT1 (Saccharomyces cerevisiae) conventional actin (yeast) 86.7 18 2 0 0 90.0 100.0
ACTR2 (Homo sapiens) cytoplasmic ARP (Arp2/3 complex) 48.3 17 2 1 0 85.0 95.0
ACTR3 (Homo sapiens) cytoplasmic ARP (Arp2/3 complex) 40.8 14 5 1 0 70.0 95.0
ACTL6A (Homo sapiens) nuclear ARP (SWI/SNF-family) 40.5 9 3 8 0 45.0 60.0
ACTR5 (Homo sapiens) nuclear ARP (INO80 complex) 32.8 8 1 11 0 40.0 45.0
ACTR8 (Homo sapiens) nuclear ARP (INO80 complex) 24.3 10 5 5 0 50.0 75.0
ARP9 (Saccharomyces cerevisiae) nuclear ARP (yeast RSC/SWI-SNF) 23.5 7 3 10 0 35.0 50.0
ARP7 (Saccharomyces cerevisiae) nuclear ARP (yeast RSC/SWI-SNF) 25.9 7 4 9 0 35.0 55.0

protomer-protomer interface - 74 positions: [38, 39, 40, 41, 42, 43, 44, 45, 49, 50, 53, 60, 61, 62, 63, 64, 65, 66, 110, 111, 112, 113, 114, 139, 142, 143, 147, 148, 165, 166, 167, 168, 169, 170, 171, 172, 173, 191, 194, 195, 196, 197, 202, 203, 204, 205, 208, 241, 242, 243, 244, 245, 246, 267, 268, 269, 270, 283, 286, 287, 288, 289, 290, 291, 294, 322, 324, 325, 346, 349, 351, 352, 355, 375]

protein role % id to whole structure seq identical similar different gap % identical % id+sim
ACTL7B (Homo sapiens) query (human ACTL7B) 42.9 26 13 34 1 35.1 52.7
Actl7b (Mus musculus) ACTL7 clade: mouse ortholog 43.5 25 13 35 1 33.8 51.4
ACTL7A (Homo sapiens) ACTL7 clade: human paralog 43.2 31 12 31 0 41.9 58.1
Actl7a (Mus musculus) ACTL7 clade: mouse paralog 43.7 30 12 32 0 40.5 56.8
ACTB (Homo sapiens) conventional actin 93.3 72 2 0 0 97.3 100.0
ACTA1 (Homo sapiens) conventional actin 100.0 74 0 0 0 100.0 100.0
ACT1 (Saccharomyces cerevisiae) conventional actin (yeast) 86.7 62 11 1 0 83.8 98.6
ACTR2 (Homo sapiens) cytoplasmic ARP (Arp2/3 complex) 48.3 32 11 31 0 43.2 58.1
ACTR3 (Homo sapiens) cytoplasmic ARP (Arp2/3 complex) 40.8 22 11 41 0 29.7 44.6
ACTL6A (Homo sapiens) nuclear ARP (SWI/SNF-family) 40.5 30 12 32 0 40.5 56.8
ACTR5 (Homo sapiens) nuclear ARP (INO80 complex) 32.8 20 9 40 5 27.0 39.2
ACTR8 (Homo sapiens) nuclear ARP (INO80 complex) 24.3 23 17 34 0 31.1 54.1
ARP9 (Saccharomyces cerevisiae) nuclear ARP (yeast RSC/SWI-SNF) 23.5 15 15 35 9 20.3 40.5
ARP7 (Saccharomyces cerevisiae) nuclear ARP (yeast RSC/SWI-SNF) 25.9 17 15 36 6 23.0 43.2

Per-position nucleotide contacts in ACTL7B (8A2S numbering -> ACTL7B): G13->G57, S14->S58, G15->Q59, L16->Y60, K18->K62, Q137->Q181, G156->G200, D157->H201, G158->G202, V159->V203, G182->G226, R210->E253, K213->K256, E214->K257, G301->G343, G302->G344, T303->C345, M305->M347, Y306->L348, K336->K377

Interface contacts broken into contiguous segments (segments are derived from the contact positions themselves, not from named literature regions):

segment n actin residues ACTL7B residues identical ACTL7B identical ACTL7A identical ACTB
38-45 8 PRHQGVMV RCPEAADA 0/8 1/8 8/8
49-50 2 QK RK 1/2 1/2 2/2
53 1 Y L 0/1 0/1 1/1
60-66 7 SKRGILT NTEAPLK 1/7 1/7 7/7
110-114 5 LNPKA LSPSS 2/5 2/5 5/5
139 1 V L 0/1 0/1 1/1
142-143 2 LY IY 1/2 1/2 2/2
147-148 2 RT KT 1/2 2/2 2/2
165-173 9 IYEGYALPH ISEGDVLPG 5/9 7/9 9/9
191 1 K Q 0/1 0/1 1/1
194-197 4 TERG NEAG 2/4 1/4 4/4
202-205 4 TTAE TDDH 1/4 1/4 4/4
208 1 I I 1/1 1/1 1/1
241-246 6 ELPDGQ ELPDGK 5/6 4/6 6/6
267-270 4 IGME AGST 1/4 1/4 3/4
283 1 M G 0/1 0/1 1/1
286-291 6 DIDIRK DTGFKE 1/6 2/6 5/6
294 1 Y A 0/1 0/1 1/1
322-325 3 PTM PDS 1/3 2/3 3/3
346 1 L L 1/1 1/1 1/1
349-352 3 LTF LAF 2/3 2/3 3/3
355 1 M L 0/1 0/1 1/1
375 1 F - 0/1 1/1 1/1