"We show that the response regulatory protein CbrB, an activator of σ(N) -dependent promoters, directly controls the expression of the small RNAs CrcZ and CrcY in P. putida."

"Thus, CbrAB constitute a global signal transduction pathway integrated in a higher regulatory network that also controls catabolite repression through the expression of the two regulatory RNAs CrcZ and CrcY."

"CbrB phosphorylation is necessary at physiological activation conditions, but a higher dose of the protein allows in vitro transcriptional activation in its non-phosphorylated form."

"determined that it directly controls the expression of at least 61 genes"

"the implication of three independent non-palindromic subsites with a variable spacing in three different targets; CrcZ, CrcY and operon PP2810-13 in the CbrAB activation."

"CbrB is a quite peculiar σN-dependent activator since it is barely dependent on phosphorylation for transcriptional activation."

"CbrB is involved in coordination with the nitrogen control system activator, NtrC, in the uptake and assimilation of several amino acids."

"CbrB affects other carbon utilization pathways and a number of apparently unrelated functions, such as chemotaxis, stress tolerance and biofilm development."

"we propose that CbrB is a high-ranked element in the regulatory hierarchy of P. putida that directly or indirectly controls a variety of metabolic and behavioural traits required for adaptation to changing environmental conditions."

"We propose that CrcZ and CrcY act in concert, sequestering and modulating the levels of free Crc according to metabolic conditions."

"The CbrA/CbrB two-component system activated crcZ transcription, but had little effect on crcY."

"Also, two additional point mutations in lapG and lapD have been detected in MPO406 by whole genome sequencing. Combination of these effects provides a robust biofilm overproducing phenotype."

"it is shown that the histidine kinase can autophosphorylate and transfer the phosphoryl group to the response regulator CbrB."

"CbrA/CbrB regulates carbon utilization and together with NtrB/NtrC ensures a balanced carbon/nitrogen relationship"

"CbrB can directly regulate expression of different σN dependent catabolic pathways, e.g., the histidine utilization (hut) operon"

"**Primary biological role**: **CbrB is a transcriptional regulatory response regulator that activates σ54-dependent promoters**, most prominently the **crcZ (and crcY) sRNA expression program**, thereby modulating Hfq/Crc-mediated CCR and nutrient adaptation."

"**Not an enzyme/transporter**: no evidence indicates that CbrB catalyzes a biochemical conversion or transports a substrate. Rather, its central “activity” is regulatory—binding/activating promoters and coupling metabolism to CCR via small RNAs."

"Biochemical work shows that **CbrA autophosphorylates and transfers the phosphoryl group to CbrB**, supporting the canonical two-component phosphorelay."

"Using a **PcrcZ::luxCDABE** reporter, **ΔcbrB** mutants did not express crcZ unless complemented with plasmid-borne **cbrB**, demonstrating that CbrB is required for crcZ activation under inducing conditions (e.g., histidine as carbon source)."

"An independent genome-scale fitness study explicitly labels **cbrB (PP_4696)** as a **σ54-dependent response regulator** and links it to central carbon metabolism and amino-acid uptake in pseudomonads."

"CbrB is a soluble, cytoplasmic response regulator (transcription factor-like) that controls transcription. It is activated by phosphorylation by the sensor kinase CbrA, which is anchored in the **inner membrane** via transmembrane segments (CbrB itself is not membrane-bound)."

"No retrieved evidence demonstrates that **PP_4696/cbrB is synonymous with the alginate biosynthesis regulator AlgB**; therefore, they should be treated as **distinct annotations** unless a sequence-level cross-reference proves equivalence."