Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Automatic Gene Ontology annotation based on Rhea mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
The pachytene checkpoint in S. cerevisiae depends on Swe1-mediated phosphorylation of the cyclin-dependent kinase Cdc28.
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The pachytene (meiotic recombination) checkpoint requires Swe1, which phosphorylates and inactivates Cdc28; swe1 deletion lets arrested mutants sporulate.
"In S. cerevisiae, this checkpoint requires Swe1, which phosphorylates and inactivates the cyclin-dependent kinase Cdc28."
Septin-dependent assembly of a cell cycle-regulatory module in Saccharomyces cerevisiae.
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Swe1p is found in the nucleus and at the daughter side of the bud neck; neck localisation requires septins, Hsl1p and Hsl7p, and stabilised Swe1p accumulates in the nucleus.
"In budded wild-type cells, Swe1p was detected only in the nucleus (12% of the cells examined), only at the neck (23% of the cells), or at both locations (39% of the cells)"
A comprehensive two-hybrid analysis to explore the yeast protein interactome.
Cdc5 interacts with the Wee1 kinase in budding yeast.
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Cdc5 interacts with Swe1 (two-hybrid, residues 173-819 of Swe1); Cdc5 overproduction modifies Swe1 and suppresses Swe1-dependent phenotypes.
"Our work shows that Cdc5, the Polo kinase in budding yeast, interacts with Swe1."
Systematic identification of protein complexes in Saccharomyces cerevisiae by mass spectrometry.
Systematic identification of pathways that couple cell growth and division in yeast.
The yeast kinase Swe1 is required for proper entry into cell cycle after arrest due to ribosome biogenesis and protein synthesis defects.
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swe1Δ delays cell-cycle re-entry after G1 arrest of ribosome-biogenesis mutants, independently of Cdc28 Tyr19 phosphorylation.
"Our data suggest that Swe1 is required for timely entry into cell cycle after a G1 arrest caused by impairment in pre-60S biogenesis and in protein synthesis."
Localization of proteins that are coordinately expressed with Cln2 during the cell cycle.
Cdk1-dependent regulation of the mitotic inhibitor Wee1.
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Cdk1 phosphorylation of Swe1 activates Swe1 and is required for a stable Swe1-Cdk1 complex that keeps Cdk1 inhibited; further phosphorylation releases Cdk1.
"Phosphorylation of Swe1 by Cdk1 activates Swe1 and is required for formation of a stable Swe1-Cdk1 complex that maintains Cdk1 in the inhibited state."
Global analysis of protein phosphorylation in yeast.
High-quality binary protein interaction map of the yeast interactome network.
A global protein kinase and phosphatase interaction network in yeast.
Regulation of Sphingolipid Biosynthesis by the Morphogenesis Checkpoint Kinase Swe1.
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swe1Δ cells are myriocin-hypersensitive owing to reduced LCB synthesis; deletion of ORM1/ORM2 or phytosphingosine rescues; Swe1 proposed to derepress SPT via Orm2.
"Deletion of the Swe1 kinase renders mutant cells sensitive to serine palmitoyltransferase inhibition due to impaired sphingoid long-chain base synthesis."
Polo-like kinase Cdc5 orchestrates Cdk1 regulation via Swe1 and Mih1 during meiotic prophase I exit.
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Swe1 is required for maintenance, not activation, of the meiotic recombination checkpoint; Cdc5 drives Swe1 degradation independently of CDK at prophase-I exit.
"We show that Swe1 is required for checkpoint maintenance but not activation."
Properties of Saccharomyces cerevisiae wee1 and its differential regulation of p34CDC28 in response to G1 and G2 cyclins.
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Swe1 is the S. cerevisiae Wee1 homologue; Swe1 immunoprecipitates tyrosine-phosphorylate and inactivate Clb2-Cdc28 but not Cln2-Cdc28, and SWE1 overexpression arrests cells in G2.
"Swe1 immunoprecipitates were capable of tyrosine phosphorylating and inactivating p34CDC28 complexed with Clb2, a G2-type cyclin, but not p34CDC28 complexed with Cln2, a G1-type cyclin, consistent with the inability of Swe1 overexpression to inhibit the G1/S transition."
Spindle pole body separation in Saccharomyces cerevisiae requires dephosphorylation of the tyrosine 19 residue of Cdc28.
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SWE1 overexpression, like the phosphomimetic cdc28-E19 allele, prevents spindle pole body separation, implying that Tyr19 dephosphorylation promotes SPB separation.
"We also find that the overexpression of SWE1, the budding-yeast homolog of wee1, also leads to a failure to segregate SPBs."
Cdc28 tyrosine phosphorylation and the morphogenesis checkpoint in budding yeast.
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The morphogenesis checkpoint delay of nuclear division in unbudded cells requires SWE1 and is controlled by SWE1/MIH1 dosage acting on Cdc28 Tyr19.
"We show that the ability of this checkpoint to delay nuclear division requires the SWE1 gene, encoding a protein kinase that inhibits the master cell cycle regulatory kinase Cdc28."
A morphogenesis checkpoint monitors the actin cytoskeleton in yeast.
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Actin cytoskeleton perturbation (mutants or latrunculin-A) triggers a Swe1p-dependent, Cdc28p tyrosine-phosphorylation-mediated delay of nuclear division.
"Thus, myo2-66 and tpm1Δ mutants experienced a cell cycle delay resulting from Cdc28p tyrosine phosphorylation (reversible by Swe1p elimination or Mih1p overexpression)."
Falcon deep research for SWE1
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Independent literature synthesis identifying Swe1 as the morphogenesis-checkpoint effector acting through Cdc28 Tyr19 phosphorylation.
"SWE1 is required for the normal delay in nuclear division in cells unable to form a bud, whereas budded cells are much less dependent on Cdc28 Tyr19 phosphorylation for ordinary cell-cycle timing."