PANTHER family review PTHR22974: IBA propagation assessment for mph1
Gene Ontology annotation through association of InterPro records with GO terms
Gene Ontology annotation based on Enzyme Commission mapping
Annotation inferences using phylogenetic trees
Automatic Gene Ontology annotation based on Rhea mapping
Electronic Gene Ontology annotations created by ARBA machine learning models
ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe.
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Genome-wide YFP tagging localized Mph1 to the nucleus and cytosol.
"we determined the localization of 4,431 proteins, corresponding to approximately 90% of the fission yeast proteome, by tagging each ORF with the yellow fluorescent protein."
Centromere-tethered Mps1 pombe homolog (Mph1) kinase is a sufficient marker for recruitment of the spindle checkpoint protein Bub1, but not Mad1.
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Kinetochore-localized Mph1 is sufficient to recruit Bub1 and to arrest cells in a checkpoint-dependent manner; Mad1 association requires an additional condition.
"We propose that Mph1 is a sufficient marker for recruitment of Bub1. Mad1, in contrast, requires an additional condition/component for stable association with kinetochores."
Kinase activity of fission yeast Mph1 is required for Mad2 and Mad3 to stably bind the anaphase promoting complex.
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Mph1 is an active kinase (phosphorylates itself and Mad2 in vitro); D459A abolishes activity; kinase activity is required for the SAC and MCC-APC/C binding.
"a kinase-dead allele of the fission yeast MPS1 homolog (Mph1) is checkpoint defective and that levels of APC/C-associated Mad2 and Mad3 are dramatically reduced in this mutant."
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Mph1 has chromosome segregation functions independent of extrinsic spindle perturbation.
"These experiments demonstrate that Mph1 kinase has important chromosome segregation function(s), even when there are no extrinsic perturbations of spindle microtubules."
Phosphodependent recruitment of Bub1 and Bub3 to Spc7/KNL1 by Mph1 kinase maintains the spindle checkpoint.
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Mph1 phosphorylates Spc7 MELT-motif threonines (e.g. T453, T507) to recruit Bub1-Bub3 to kinetochores and maintain the SAC.
"phosphorylation of the conserved MELT motifs in Spc7 by Mph1 (Mps1) recruits Bub1 and Bub3 to the kinetochore and that this is required to maintain the SAC signal."
MPS1/Mph1 phosphorylates the kinetochore protein KNL1/Spc7 to recruit SAC components.
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Mph1 at unattached kinetochores phosphorylates Spc7 MELT repeats, creating a mark crucial for SAC activation and chromosome biorientation.
"MPS1/Mph1 kinase locating at the unattached kinetochores initially creates a mark, which is crucial for SAC activation and chromosome bi-orientation."
Mph1 kinetochore localization is crucial and upstream in the hierarchy of spindle assembly checkpoint protein recruitment to kinetochores.
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Mph1 (with Ark1) is at the top of the kinetochore SAC recruitment hierarchy; loss of Mph1 kinetochore enrichment abolishes SAC signaling and forced recruitment restores it.
"a three-layered hierarchy with Ark1 and Mph1 on top, Bub1 and Bub3 in the middle, and Mad3 as well as the Mad1-Mad2 complex at the lower end of the hierarchy."
A knockout screen for protein kinases required for the proper meiotic segregation of chromosomes in the fission yeast Schizosaccharomyces pombe.
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mph1Delta causes meiosis I homolog non-disjunction; Mph1 is required for efficient homolog disjunction during meiosis I.
"Mph1, a member of the Mps1 family of spindle assembly checkpoint kinases, is required to prevent meiosis I homolog non-disjunction."
Mps1Mph1 Kinase Phosphorylates Mad3 to Inhibit Cdc20Slp1-APC/C and Maintain Spindle Checkpoint Arrests.
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Mad3 is a direct Mph1 substrate; Mph1-dependent modification of Mad3 and Mad2 stabilizes MCC-APC/C binding and maintains the checkpoint arrest.
"Mps1(Mph1) kinase-dependent modifications of Mad3 and Mad2 act in a concerted manner to maintain spindle checkpoint arrests."
Bub3-Bub1 Binding to Spc7/KNL1 Toggles the Spindle Checkpoint Switch by Licensing the Interaction of Bub1 with Mad1-Mad2.
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Mph1 phosphorylates Spc7 MELT motifs to recruit Bub1/Bub3/Mad3; multisite Bub3 binding to the MELT array toggles the checkpoint by licensing Bub1-Mad1-Mad2 interaction.
"Mph1 (Mps1) phosphorylates multiple conserved MELT motifs in the Spc7 (Spc105/KNL1) protein to recruit Bub1, Bub3, and Mad3 (BubR1) to kinetochores"
Meikin-associated polo-like kinase specifies Bub1 distribution in meiosis I.
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In meiosis I, the meikin(Moa1)-Plo1 module phosphorylates Spc7 to accumulate Bub1 at kinetochores, supporting Sgo1-dependent centromeric cohesion protection.
"recruits Plo1 (polo-like kinase) to the kinetochores and phosphorylates Spc7 (KNL1) to accumulate Bub1"
Mph1, a member of the Mps1-like family of dual specificity protein kinases, is required for the spindle checkpoint in S. pombe.
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mph1 is a spindle checkpoint gene required for checkpoint activation in response to spindle defects, acting upstream of mad2; unlike S. cerevisiae Mps1 it is not required for SPB duplication.
"mph1 functions upstream of mad2, a previously characterized component of the spindle checkpoint."