ARO1 (YDR127W, P08566) notes

Pathway context: shikimate/chorismate pathway steps 2-6 (YeastPathways ARO-PWY-1); EC 4.2.3.4, 4.2.1.10, 1.1.1.25, 2.7.1.71, 2.5.1.19.

• Pentafunctional AROM, 1588 aa, mosaic of E. coli-like domains PMID:2825635; domain order DHQS - EPSPS - SK - DHQase(type I) - SDH [UniProt:P08566].
• Cloned ARO1 overproduces all five activities and complements E. coli aroA/B/D/E [PMID:6355828 "express 5 to 12 times the normal level of the five AROl enzyme activities"; "fragment also complemented aroA, aroB, aroD, and aroE mutants of Escherichia coli"].
• GCN4 general control PMID:2848727.
• aro1/aro2 homozygotes fail to sporulate PMID:361708.
• Zn: 2 Zn2+/subunit (HAMAP) [UniProt:P08566]; zinc-proteome RCA kept non-core.
• Curation: three HTP protein-binding IPIs (Gavin 2002/2006, Hsp90 map) REMOVEd as uninformative; "chorismate metabolic process" RCA (superpathway) MODIFY -> chorismate biosynthetic process.
• Module note: AROM fusion is fungal/protist-specific; bacteria/plants use separate AroB, AroD/DHQ-SDH, AroE, AroK, AroA polypeptides. Type I DHQase (not type II) in yeast.