Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot keyword mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Automatic assignment of GO terms using logical inference, based on on inter-ontology links
Electronic Gene Ontology annotations created by ARBA machine learning models
Large-scale proteomics and phosphoproteomics of urinary exosomes.
Insulin receptor tyrosine kinase substrate links the E. coli O157:H7 actin assembly effectors Tir and EspF(U) during pedestal formation.
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IRTKS serves as an essential adapter linking bacterial effectors Tir and EspF(U) to trigger actin assembly. The IMD domain binds Tir while the SH3 domain binds EspF(U). Genetic depletion of IRTKS blocks pedestal formation.
"Ectopic expression of either the IRTKS SH3 domain or the IMD, or genetic depletion of IRTKS, blocked pedestal formation"
Recognition of tandem PxxP motifs as a unique Src homology 3-binding mode triggers pathogen-driven actin assembly.
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NMR structure reveals IRTKS SH3 domain binds tandem PxxP motifs with uniquely high affinity. This SH3-mediated interaction is required for actin pedestal formation during bacterial infection.
"EspF(U)-IRTKS interaction is among the highest affinity natural SH3 ligands. Our complex structure reveals a unique type of SH3 interaction based on recognition of tandem PxxP motifs"
Enterohaemorrhagic Escherichia coli exploits a tryptophan switch to hijack host f-actin assembly.
In-depth proteomic analyses of exosomes isolated from expressed prostatic secretions in urine.
E-cadherin interactome complexity and robustness resolved by quantitative proteomics.
The EHEC-host interactome reveals novel targets for the translocated intimin receptor.
A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
A reference map of the human binary protein interactome.
Dual proteome-scale networks reveal cell-specific remodeling of the human interactome.
OpenCell: Endogenous tagging for the cartography of human cellular organization.
RHOF binds effectors at the plasma membrane
Characterisation of IRTKS, a novel IRSp53/MIM family actin regulator with distinct filament bundling properties.
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IRTKS directly binds F-actin and promotes actin bundle formation. The C-terminal extension interacts with actin filaments and modulates IMD organising activity.
"Expression of IRTKS induces clusters of short actin bundles rather than filopodia-like protrusions"
Deep research review of BAIAP2L1/IRTKS function
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I-BAR proteins including IRTKS recognize ~100 nm membrane evaginations generated by mechanical stretch, recruiting Rac1-WAVE-Arp2/3 to polymerize actin and re-flatten the membrane.
"I-BAR proteins (including IRTKS) recognize ~100 nm membrane evaginations after stretch/compression, recruiting Rac1-WAVE/Arp2/3 to polymerize actin"
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IRTKS undergoes LLPS, infiltrates HP1alpha condensates, and recruits Ubc9 to SUMOylate/stabilize HP1alpha. IRTKS loss leads to heterochromatin erosion, cGAS-STING activation, and cellular senescence.
"IRTKS undergoes liquid-liquid phase separation, infiltrates HP1alpha condensates, recruits Ubc9 to SUMOylate/stabilize HP1alpha"