Combined Automated Annotation using Multiple IEA Methods

file:PSEPK/imuB/imuB-uniprot.txt

UniProtKB entry for imuB (Q88I83)

Q88I83 has an IMS/UmuC-like polymerase fold and is encoded between ImuA and the reviewed DnaE2 polymerase.

file:PSEPK/imuB/imuB-deep-research-openscientist.md

OpenScientist research report for imuB (Q88I83)

The report supports a catalytically inactive polymerase-like scaffold role using ortholog interaction data, conserved cassette structure, and loss of key catalytic carboxylates.

PMID:20615954

Essential roles for imuA'- and imuB-encoded accessory factors in DnaE2-dependent mutagenesis in Mycobacterium tuberculosis.

Mycobacterial ImuB interacts with ImuA-prime, DnaE2 and the beta clamp; its noncatalytic polymerase-like architecture supports an adaptor interpretation by analogy for PSEPK.

"Yeast two-hybrid analyses indicate that ImuB interacts with both ImuA' and DnaE2, as well as with the beta-clamp."

PMID:17331811

Study of involvement of ImuB and DnaE2 in stationary-phase mutagenesis in Pseudomonas putida.

P. putida ImuB promotes accumulation of stationary-phase mutants.

"accumulation of stationary-phase mutants up to two-fold."

PMID:28118378

DNA Polymerases ImuC and DinB Are Involved in DNA Alkylation Damage Tolerance in Pseudomonas aeruginosa and Pseudomonas putida.

The native experiments use PaW85 derivatives, described as isogenic to the KT2440 reference strain.

"All Pseudomonas putida strains are derivatives of PaW85 [54], which is isogenic to KT2440 [55]."
Individual imuA, imuB and imuC deletions compromise alkyl-damage tolerance in an AlkA-deficient background; ImuB complementation addresses polarity.

"ImuB and ImuA are both required for the involvement of ImuC in the DNA alkyl damage tolerance in pseudomonads."
ImuC promotes MMS-induced mutagenesis in the tested conditions, despite older antimutator results under different stresses.

"indicating that ImuC is responsible for the MMS mutability in P. putida"