Gene Ontology annotation through association of InterPro records with GO terms
Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniPathway vocabulary mapping
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Electronic Gene Ontology annotations created by ARBA machine learning models
Combined Automated Annotation using Multiple IEA Methods
Cloning and expression of a human choline/ethanolaminephosphotransferase: synthesis of phosphatidylcholine and phosphatidylethanolamine.
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Human CEPT1 (hCEPT1p, 416 aa) is a dual-specificity phosphotransferase that uses both CDP-choline and CDP-ethanolamine as phosphobase donors to diacylglycerol, synthesizing both PC and PE in vitro and in vivo; it catalyzes the final step of the Kennedy pathway.
"In vitro, hCEPT1p displayed broad substrate specificity, utilizing both CDP-choline and CDP-ethanolamine as phosphobase donors to a broad range of diacylglycerols, resulting in the synthesis of both PtdCho and PtdEtn."
The major sites of cellular phospholipid synthesis and molecular determinants of Fatty Acid and lipid head group specificity.
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Human CEPT1 localizes to the ER and nuclear membranes (not the Golgi, unlike the paralog CPT1); Gly156 governs its dual CDP-alcohol specificity.
"confirmed that CPT1 was found in the Golgi and CEPT1 was found in both the endoplasmic reticulum and nuclear membranes."
A reference map of the human binary protein interactome.
Structural basis for catalysis of human choline/ethanolamine phosphotransferase 1.
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Cryo-EM structures of human CEPT1 (dimer, 10 TMs per protomer) with CDP-choline; CEPT1 catalyzes the last step of PC and PE synthesis in the Kennedy pathway, prefers Mg2+ (Mn2+ ~50%), requires DAG, and depends on the catalytic aspartate D154.
"Choline/ethanolamine phosphotransferase 1 (CEPT1) catalyzes the last step of the biosynthesis of PC and PE in the Kennedy pathway by transferring the substituted phosphate group from CDP-choline/ethanolamine to diacylglycerol."
CDP-Cho and DAG are converted to PC by CEPT1 at the ER membrane
CDP-ETA and DAG are converted to PE by CEPT1/EPT1
Cloning, genomic organization, and characterization of a human cholinephosphotransferase.
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Distinguishes CEPT1 (dual choline/ethanolamine specificity) from the choline-specific paralog CPT1; source characterizing the ether-lipid / platelet-activating-factor precursor choline phosphotransferase side activity.
"This contrasted with our previously cloned human choline/ethanolaminephosphotransferase cDNA that was demonstrated to code for a dual specificity choline/ethanolaminephosphotransferase."