Annotation inferences using phylogenetic trees
Gene Ontology annotation based on UniProtKB/Swiss-Prot Subcellular Location vocabulary mapping, accompanied by conservative changes to GO terms applied by UniProt
Gene Ontology annotation based on curation of immunofluorescence data
Electronic Gene Ontology annotations created by ARBA machine learning models
Genetic and structural characterization of the human mitochondrial inner membrane translocase.
Novel raf kinase protein-protein interactions found by an exhaustive yeast two-hybrid analysis.
Tim50, a component of the mitochondrial translocator, regulates mitochondrial integrity and cell death.
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Human TIMM50 is a mitochondrial inner membrane protein, anchored by its N-terminal transmembrane segment with the CTD-like domain facing the intermembrane space.
"indicating that it is likely anchored to the inner mitochondrial membrane via its N-terminal TM domain"
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TIMM50 co-immunoprecipitates with TIMM23 from HeLa mitochondria, the basis for naming it the human Tim50 ortholog.
"The 40 kDa protein coprecipitated with anti-Tim23 monoclonal antibody but not with the control T7 monoclonal antibody"
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Phosphatase activity was assayed only with GST fusions purified from E. coli: full length and the C-terminal region (110-353) hydrolysed pNPP dose-dependently, the N-terminal fragment did not. No catalytic-site mutant was tested.
"both the full-length human Tim50 and the isolated CTR (residues 110–353), but not the MTS (residues 1–50), catalyzed the conversion of pNPP to pNP and inorganic phosphate in a dose-dependent manner"
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The activity had an acidic pH optimum (4.0-6.0) and fell sharply at basic pH.
"The human Tim50 phosphatase activity displayed a bell-shaped pH profile with an optimum activity around pH 4.0–6.0. Phosphatase activity declined sharply at basic pH"
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The fusions also dephosphorylated phosphotyrosine peptides and, more weakly, a phosphothreonine peptide.
"Human Tim50 and its isolated CTR also exhibited activity toward Ser/Thr(P)-peptide RRA(pT)VA"
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No physiological substrate was identified; the authors only speculate about Bcl-2 family proteins, and note that yeast Tim50 lacks the activity.
"It is tempting to speculate that in addition to its translocase activity, human Tim50 might also function as a phosphatase for certain mitochondrial proteins"
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TIMM50 was first isolated in a yeast two-hybrid screen with the DR5 death domain, but no interaction between the endogenous proteins could be detected; the paper does not concern interleukin-2 receptor.
"subsequent co-immunoprecipitation assays with extracts from several human cell lines before or after stimulation with TRAIL failed to detect any interactions between the endogenous 40 kDa protein and endogenous DR4 or DR5"
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siRNA depletion of TIMM50 sensitized human cells to UV- and staurosporine-induced cytochrome c release and apoptosis, and zebrafish morphants showed neurodegeneration and cell death.
"and apoptosis in human Tim50-depleted cells compared with control cells"
Tim50a, a nuclear isoform of the mitochondrial Tim50, interacts with proteins involved in snRNP biogenesis.
Integrative analysis of kinase networks in TRAIL-induced apoptosis provides a source of potential targets for combination therapy.
ROMO1 is a constituent of the human presequence translocase required for YME1L protease import.
Extensive rewiring of the EGFR network in colorectal cancer cells expressing transforming levels of KRAS(G13D).
Ttm50 facilitates calpain activation by anchoring it to calcium stores and increasing its sensitivity to calcium.
Quantitative high-confidence human mitochondrial proteome and its dynamics in cellular context.
Interactome dynamics of RAF1-BRAF kinase monomers and dimers.
Reduced Protein Import via TIM23 SORT Drives Disease Pathology in TIMM50-Associated Mitochondrial Disease.
D-x-D-x-(T/V) motif scan of Tim50 proteins
Falcon deep research report for human TIMM50